ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
A reverse phase high performance liquid chromatographic method was used to separate the o-phthaldialdehyde derivatives of of amino acids from food protein hydrolysates. Comparison of hydrolysis with hydrochloric, methanesulfonic and mercaptoethanesulfonic acids revealed that the latter could not be used because it interfered with derivatization. Methanesulfonic acid is preferable to hydrochloric acid since it protects the tryptophan in samples low in carbohydrates. Hydrolysates were prepurified with a Sep Pak C18 cartridge prior to derivatization. A linear gradient was used for combining the binary solvent system composed of acetonitrile and phosphate buffer. The derivatives were measured fluorometrically and histamine was used as an internal standard. Results obtained with this method were similar to those obtained with an ion exchange chromatography except that threonlne and glycine coeluted and cysteine, cystine and proline were not detected.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.1982.tb11098.x
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