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  • 1
    Electronic Resource
    Electronic Resource
    Selection response and efficiency of doubled-haploid recurrent selection in a cross-fertilized species (1988)
    Springer
    Theoretical and applied genetics 75 (1988), S. 410-414 
    Details
    ISSN: 1432-2242
    Keywords: Computer simulation ; Doubled haploids ; Response to selection ; Mass selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Computer simulation was used to compare the simulated response to doubled-haploid (DH) mass selection with the response predicted by mathematical formulae. The efficiency of DH versus diploid mass selection in a cross-fertilized species was also studied by means of theoretical consideration and computer simulation. Simulated gain was in agreement with the predicted gain in the DH population under both additive and complete dominance models. The simulated variance of response to DH mass selection was close to the predicted variance at both the 5% and 25% selection regimes under additive and complete dominance models. The efficiency of DH over diploid mass selection was shown to be dependent upon the allelic frequency, the degree of dominance, and the amount of environmental variance. In theory the efficiency can range from zero to infinity, but in reality it should be greater than one. The efficiency ranges from √2 to 2 in the absence of dominance; it can be greater than two only in the presence of dominance and a small environmental variance. The variance of response to DH mass selection can be smaller than or up to twice as large as the variance of response to diploid mass selection. Computer simulation results agreed with the predicted efficiency of DH mass selection and with the predicted variance-of-response ratio of DH mass selection to diploid mass selection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276743
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Genetic studies of corn (Zea mays L.) anther culture response (1990)
    Springer
    Theoretical and applied genetics 80 (1990), S. 459-464 
    Details
    ISSN: 1432-2242
    Keywords: Corn ; Anther culture ; Genetic studies ; Generation mean analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Anthers of two maize (Zea mays L.) inbred lines, DBTS (P1) and B73 (P2), their F1, F2 and first backcross generations — F1 x DBTS (B1), and F1 x B73 (B2) — were float cultured in YP medium to study the inheritance of corn anther culturability using generation mean analysis. Significant effects of generation were observed for the three traits measured: anther response (%), frequency of embryos (%) and anther productivity. Variation among the generations was similar for anther response and frequency of embryos: no significant differences were found among the P1, F1, F2 and B1 means, but the means of P2 and B2 were significantly lower than those of the other generations. For anther productivity, the F2 generation tended to have a slightly higher tendency for multiple embryo formation. A simple additive-dominance model was adequate in explaining the inheritance of anther response and frequency of embryos, but digenic epistasis (additive x dominance) was involved in the inheritance of anther productivity. Additive genetic variance was higher than non-additive genetic variance for all the traits; however, only environmental variance was significant. Narrow-sense heritability estimates were 65% and 75% for anther response and frequency of embryos, respectively. Significant inter-plant variation was observed within generations, even for the inbred line DBTS, but isozymic analysis involving five enzyme loci did not reveal any genotypic variability within the inbred lines DBTS and B73.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226745
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Increased induction of microspore embryos following manipulation of donor plant environment and culture temperature in corn (Zea mays L.) (1992)
    Springer
    Plant cell, tissue and organ culture 28 (1992), S. 87-90 
    Details
    ISSN: 1573-5044
    Keywords: ELS (embryo-like structures) ; greenhouse ; growth chamber ; Seneca-60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Microspore cultures were initiated from the North American sweet corn hybrid, ‘Seneca 60’. Donor plants were grown under two environments. One treatment comprised plants that matured completely in the greenhouse (GH) (28°C/23°C: day/night), while in a second treatment donor plants were isolated and divided into two treatment sets: cultured directly at 25°C, or given a heat treatment of 32°C for 10 days. Greenhouse-grown plants produced fewer embryo-like structures (ELS) than growth chambertreated plants regardless of the culture temperature treatment. If the microspores isolated from GC plants were subsequently provided with the initial high culture temperature, the number of ELS that could be recovered was more than doubled compared to the cultures incubated at 25°C continuously. The high culture temperature treatment also resulted in a higher quality of ELS (more compact), which led to a higher frequency of ELS that survived and were subsequently transferred to regeneration medium. However, while plant regeneration and subsequent selfed seeds were obtained, the frequency was very low and further research is required in this area.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039919
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    Paper (German National Licenses)
    Fulltext
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