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  • 1
    Electronic Resource
    Electronic Resource
    Crystal growth in syndiotactic poly(vinyl alcohol) hydrogels (1987)
    Springer
    Colloid & polymer science 265 (1987), S. 810-814 
    Details
    ISSN: 1435-1536
    Keywords: Crystal growth ; syndiotactic poly(vinyl alcohol) ; hydrogels ; Liesegang rings ; diffusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Aqueous solutions of syndiotacticity-rich poly(vinyl alcohol) (s-PVA) form gels easily. The optimum condition of growth of the calcium tartrate crystal formed by diffusing calcium chloride into hydrogels containing tartaric acid was studied with use ofs- PVA of a syndiotacticity of 56 % and a degree of polymerization of 1460. The crystal grew in the gel of the concentrations of 2 % s-PVA and of 0.5 N tartaric acid at pH=4. The relation between the formation of Liesegang rings and shear modulus of a gel was studied by diffusing silver nitrate into gels containing potassium chromate. The distance between rings decreased with increasing shear modulus of a gel in the range from 670 to 7500 dyne/cm2. The Liesegang rings were not formed for the shear modulus gel for 280 and 16200 dyne/cm2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01418457
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  • 2
    Electronic Resource
    Electronic Resource
    Cytosolic phospholipase A2, increased and activated in the eosinophils of patients with hypereosinophilic syndrome in vivo, is involved in the augmented release of leukotriene C4 (1999)
    Springer
    Inflammation research 48 (1999), S. 36-40 
    Details
    ISSN: 1420-908X
    Keywords: Key words: Cytosolic phospholipase A2 (cPLA2) — Eosinophils — A23187 — Leukotriene C4 (LTC4) — 5-Lipoxygenase (5-LO)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Objective: Eosinophils from patients with hypereosinophilic syndrome (HES) showed augmented release of leukotriene C4 (LTC4) by stimulation with A23187. In the present study, we investigated the involvement of cytosolic phospholipase A2 (cPLA2) in this phenomenon.¶Methods: Eosinophils from normal and HES donors (2.5 × 106 cells/ml) were incubated with A23187 (0.03–3 μM) for 60 min in the presence or absence of a cPLA2 inhibitor, AACOCF3. The LTC4 released from eosinophils was measured by enzyme immunoassay. Distribution of cPLA2 and 5-lipoxygenase (5-LO) proteins within the eosinophils were detected by Western immunoblotting.¶Results: The level of LTC4 released from the HES eosinophils by stimulation with A23187 was higher than that from normal eosinophils. The A23187-induced LTC4 release was inhibited by AACOCF3 in a dose-dependent manner. The amounts of cPLA2 seemed to be increased in the non-stimulated HES eosinophils by an analysis of immunoblotting. To be noticed was that cPLA2 was detected as a phosphorylated and membrane-bound form in the HES eosinophils, but not in the normal eosinophils. In contrast, localization of 5-LO within the eosinophils under A23187 stimulation was not different between normal and HES donors, while the amounts of 5-LO also seemed to be increased in the HES eosinophils.¶Conclusions: These results suggest that cPLA2, increased and activated (phosphorylated and membrane-translocated) in vivo, is involved in the augmented release of LTC4 from the HES eosinophils.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s000110050388
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  • 3
    Electronic Resource
    Electronic Resource
    Continuous preparative method of relatively large and uniform polymer beads and their application to immobilization of urease (1988)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 35 (1988), S. 1283-1288 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: An apparatus for continuous preparative method of polymer beads was investigated. Monomer droplets formed in glycerol were continuously introduced to a rotating glass tube which was filled with warmed glycerol and polymerized. Polymer beads of around 4 mm diameter were obtained. As an application of the polymer beads, urease was immobilized on the beads and their properties were evaluated. Macroreticular type of beads prepared with a mixture of maleic anhydride, styrene, and divinylbenzene showed the highest enzymatic activity among the beads tested, and the urease immobilized beads could be successfully applied for determination of blood urea nitrogen in human sera.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1988.070350514
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