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  • 1
    ISSN: 1420-908X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstracts An economic and sensitive test system for measuring random and directional migration of human neutrophils is described. The technique, based on a modified Boyden chamber equipped with a two-filter system, permits a substantial reduction of both incubation time and sample volume. The influence of various technical factors such as the neutrophil concentration in the cell suspension, the incubation time of the chambers, the test concentration of activated plasma or serum, the presence of heparin, and the procedure for separating neutrophils from human peripheral blood, was investigated. Standardized procedures for measuring and reporting neutrophil chemotaxis are proposed. The method has been used to study the significance of factors regulating neutrophil migration such as cytotaxin inactivators and neutrophil immobilizing factors (NIF). Activity of cytotaxin inactivators as assessed in undiluted serum or plasma at pH 7.4, 6.0 or 4.0 was very low. In contrast, potent neutrophil immobilizing activity was found in human serum or diluted plasma. These factors which inhibit migration were accordingly termed neutrophil immobilizing factors of plasma (NIF-P) and neutrophil immobilizing factor of serum (NIF-S). These factors are heat-stable, non-dialysable and of high molecular weight.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of clinical periodontology 10 (1983), S. 0 
    ISSN: 1600-051X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract Recently we had the opportunity 10 examine and follow up over a period of 2 years an unusual case of Papillon-Lefèvre syndrome (PLS). A 10 year old boy exhibited all symptoms typical of PLS except periodontitis and premature loss of deciduous teeth. The present report aimed at studying the functional capacity of his neutiophilic granulocytes. It integrates clinical observations, histopathological findings and results of in vitro tests, The bioptic material examined included one gingival biopsy, extracted teeth, suppurative material discharged from periodontal pockets, pus emanating from a mucosal abscess, and peripheral blood leucocytes. The neutrophils were sampled on two separate occasions in two independent laboratories and tested for a variety of functions, i.e. motility, random and directional locomotion (chemotaxis), phagocytosis, membrane potential depolarization, oxygen consumption, NBT reduction, and intracellular killing of bacteria and fungi. Findings and test data indicated that in this case of PLS, neutrophilic granulocytes behaved normally with respect to all these functions including margination in blood vessels, emigration, phagocytosis of a broad range of bacteria, degranulation of lysosomes, and intracellular destruction. The data imply that factors other than neutrophil defects may be responsible for rapidly destructive periodontitis in cases where PLS is not associated with an increased susceptibility to infection.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Venus is completely covered by a thick cloud layer, of which the upper part is composed of sulphuric acid and some unknown aerosols. The cloud tops are in fast retrograde rotation (super-rotation), but the factors responsible for this super-rotation are unknown. Here we report observations of ...
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The irreversible conversion of methane into higher hydrocarbons in Titan's stratosphere implies a surface or subsurface methane reservoir. Recent measurements from the cameras aboard the Cassini orbiter fail to see a global reservoir, but the methane and smog in Titan's atmosphere impedes ...
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The first scientific objective of the HMC is to discover and observe the comet nucleus and to attempt to verify its solid nature and the general concept of the icy conglomerate model of cometary nuclei, as proposed by Whipple1 about 36 years ago. This objective involves the determination of the ...
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Earth, moon and planets 72 (1996), S. 79-89 
    ISSN: 1573-0794
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract The icy conglomerate model introduced by Whipple more than 40 years ago has been widely accepted in cometary science because it is able to describe numerous cometary phenomena. In this model comets are described as a conglomerate of ices and dust where the ices represent the major component. However, some recent observations seem to favour dust rich comets. The purpose of this paper is to summarize the observational facts supporting the dominance of refractories in comets and to discuss the consequences of a dust dominated nucleus for cometary physics.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Earth, moon and planets 72 (1996), S. 103-112 
    ISSN: 1573-0794
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Images of comet Halley's nucleus taken by the HMC camera during the GIOT-TO encounter in 1986 show that a major part of the total dust production is localized in a few active areas which are the sources of gas-dust jets. The global dust distribution in the inner coma is dominated by two main jets roughly directed to the sun. A combination of a 1D thermal nucleus model with an axisymmetric continuum model of the jet outflow was used to investigate the properties of the inner coma. Detailed investigations show that the characteristics of the observed jets can be reproduced by outgassing from free sublimating active areas of a few km in diameter, a dust to gas ratio of 1–2.5 and a size distribution dominated by the larger grains. It is further shown that most of the observational constraints provided by the HMC data can be met simultaneously by a model of three jets superimposed on a weak background.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1573-0794
    Keywords: C/1995 O1 Hale-Bopp ; comets ; infrared spectroscopy ; ISO
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Spectra of comet C/1995 O1 (Hale-Bopp) were obtained with the Infrared Space Observatory (ISO) at medium resolution with the grating spectrometer in the photometer (PHT-S) and/or at high resolution with the short wavelength spectrometer (SWS) and long wavelength spectrometer (LWS) in April 1996 (Crovisier et al., 1996), September–October 1996 (Crovisier et al., 1997a, b) and December 1997, at distances from the Sun of 4.6, 2.9 and 3.9 AU, respectively. For the first time, high-resolution spectra of a comet covering the entire 2.4 to 200 μm spectral range were obtained. The vibrational bands of H2O, CO2 and CO are detected in emission with PHT-S. Relative production rates of 100:22:70 are derived for H2O:CO2:CO at 3 AU pre-perihelion. H2O is observed at high spectral resolution in the ν3 group of bands around 2.7 μm and the ν2 group around 6 μm with SWS, and in several rotational lines in the 100–180 μm region with LWS. The high signal-to-noise ratio of the ν3 band observed on September–October 1996 allows accurate determinations of the water rotational temperature (28 K) and of its ortho-to-para ratio(2.45 ± 0.10, which significantly differs from the high temperature limit and corresponds to a spin temperature of 25 K). Longward of 6 μm the spectrum is dominated by dust thermal continuum emission, upon which broad emission features are superimposed. The wavelengths of the emission peaks correspond to those of Mg-rich crystalline olivine (forsterite). In the September–October 1996 spectra, emission features at 45 and 65 μm and possible absorption at 2.9–3.2 μm suggest that grains of water ice were present at 3 AU from the Sun. The observations made post-perihelion in late December 1997 led to the detections of H2O, CO2 and CO at 3.9 AU from the Sun (Figures 1 and 2). The production rates were ≈3.0 × 1028,3.5 × 1028 and ≈1.5 × 1029 s-1, respectively. This corresponds to H2O:CO2:CO = 100:110:500 and confirms that at such distances from the Sun, cometary activity is dominated by sublimation of CO and CO2 rather than by H2O.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 33 (1977), S. 1386-1387 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Experiments performed by means of time lapse cinematography or the filter technique show that human serum albumin has marked chemokinetic effects on neutrophil cultured in Gey's solution. The average speed of the cells, as well as the proportion of neutrophils showing locomotion, is increased. Enhanced locomotion correlates with decreased attachment to the substratum as determined by morphological and functional criteria.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 35 (1979), S. 1669-1671 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Serum peptides containing classical anaphylatoxin (CAT) produce marked chemotactic orientation of human neutrophil granulocytes without modifying cell attachment to the substratum. Furthermore gradients of adhesion produced with gammaglobulins fail to induce morphological orientation of neutrophils. The results suggest that chemotaxis is not a special case of haptotaxis.
    Type of Medium: Electronic Resource
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