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  • 1
    Electronic Resource
    Electronic Resource
    Modified High Frequency Apparatus for Determination of Moisture in Solids (1954)
    s.l. : American Chemical Society
    Analytical chemistry 26 (1954), S. 1716-1719 
    Details
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ac60095a011
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  • 2
    Electronic Resource
    Electronic Resource
    Transynaptic transport of procion yellow in the central nervous system (1979)
    Springer
    Experimental brain research 35 (1979), S. 371-385 
    Details
    ISSN: 1432-1106
    Keywords: Transynaptic transport ; CNS ; Procion Yellow ; Guinea pig ; In vitro
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Intracellular recordings were performed in slices of the lateral geniculate body, the visual cortex, and the hippocampus of guinea pigs. After recording, Procion Yellow was iontophoretically injected. During the injection period the neurones were activated antidromically and/or transynaptically. Up to 6 h after dye injection slices were fixed in a buffered formaldehyde/sucrose solution and later histologically processed. In the lateral geniculate body, after orthodromic stimulation across the optic tract, optic tract fibres were stained secondarily. Orthodromic activation across intrageniculate connections led to secondarily stained geniculate neurones. In the visual cortex orthodromic activation of the injected neurones across white matter led to secondarily stained fibres travelling in the direction to the white matter or to secondarily stained neurones in the vicinity of the injected neurone. In the hippocampus anti- and orthodromic activation across the alveus or the mossy fibre pathway led to a secondary staining of neighbouring pyramidal neurones and to a secondary staining of fibres which where afferent to the injected neurone. All slices where Procion Yellow was detected in the extracellular space were rejected. These findings led to the conclusion that Procion Yellow must have been transported across chemical synapses from the post- to the presynaptic side. The transport occurred only if the concerned synapses were activated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236621
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  • 3
    Electronic Resource
    Electronic Resource
    The specificity of the response of preoptic-septal area neurons to estrogen: 17α-estradiol versus 17β-estradiol and the response of extrahypothalamic neurons (1977)
    Springer
    Experimental brain research 30 (1977), S. 43-52 
    Details
    ISSN: 1432-1106
    Keywords: Transport number ; Stereospecificity ; Preoptic area ; Extrahypothalamic neurons ; Estradiol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The purpose of this study was to determine the specificity of the response of medial preoptic-septal neurons (mPOA-S) to microelectrophoresed 17β-estradiol hemisuccinate (17βE2S). In vitro studies were conducted initially to determine the release of the labeled 17βE2S from multibarrel glass micropipettes. Subsequently, an isomer of 17βE2S, 17α-estradiol hemisuccinate (17αE2S), was synthesized and purified. Thirty-six mPOA-S neurons from normal cycling female rats were tested with both 17βE2S and 17αE2S. Twelve of these units responded with inhibition to 17βE2S, while none responded to 17αE2S. Furthermore, fifty extrahypothalamic (cortical, hippocampal, thalamic) neurons were tested with 17βE2S. The majority (N = 45) showed no response, three showed excitation and two inhibition to the microelectrophoresed steroid ester. These findings suggest that a specific receptor mechanism is responsible for the changes in mPOA-S unit activity, and that these effects may be important in the regulation of reproductive events.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237857
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  • 4
    Electronic Resource
    Electronic Resource
    The effects of microelectrophoretically applied estrogen, cortisol and acetylcholine on medial preoptic-septal unit activity throughout the estrous cycle of the female rat (1977)
    Springer
    Experimental brain research 30 (1977), S. 53-64 
    Details
    ISSN: 1432-1106
    Keywords: 17β-estradiol ; Microelectrophoresis ; Antidromic identification ; Preoptic activity ; Interneuron
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The technique of microelectrophoresis was utilized to study the effects of 17β-estradiol hemisuccinate (17βE2S), cortisol hemisuccinate (CS), and acetylcholine (Ach) on medial preoptic-septal (mPOA-S) neurons throughout the estrous cycle in urethane anesthetized rats. A total of 23 cells were identified antidromically as having their projections to the arcuate-median eminence region of the hypothalamus, and 18 of these were tested with 17βE2S. Only four cells showed an inhibition to the gonadal steroid, while the remaining neurons did not respond. Twelve antidromically identified (AI) neurons were tested with CS, none of which responded, and 2 out of 16 AI neurons showed an excitation to Ach. Extracellular potentials were recorded from 273 non-antidromically identified neurons, 138 of which were successfully tested with 17βE2S. Sixty-two of these responded to the steroid. The predominant response on late diestrus I was excitation, and the majority of estrogen-sensitive cells showed an inhibitory response on late diestrus 2, proestrus and estrus. A Chi-square analysis showed that the response varied significantly throughout the estrous cycle. Cortisol hemisuccinate was tested on 154 non-antidromically identified mPOA-S neurons. The response to the corticosteroid also varied significantly throughout the cycle, but further analysis determined that the response was different from that of 17βE2S. Acetylcholine was also applied to 180 mPOA-S neurons, but no significant change in the response was found from one day of the cycle to the next. It is apparent from these studies that (1) 17βE2S can bring about direct and rapid changes in the firing frequency of neurons, (2) the non-antidromically identified neuron is a site for these effects, and (3) the response differs significantly throughout the estrous cycle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237858
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  • 5
    Electronic Resource
    Electronic Resource
    Single unit recordings in the rat pineal gland: Evidence for habenulo-pineal neural connections (1980)
    Springer
    Experimental brain research 39 (1980), S. 187-192 
    Details
    ISSN: 1432-1106
    Keywords: Pineal ; Single unit recording ; Habenula stimulation ; Habenulo-pineal pathway
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Extracellular potentials were recorded in the pineal gland of urethane-anesthetized rats. Two distinct populations of excitable pineal “cells” were found, the silent “cells” which were driven by habenula stimulation and the spontaneously active cells. In the former case 17 of the responses (median latency of 1.2 ms) showed a positive-negative potential, and 6 (about 1 ms latency) showed only positive potential of 1–2 ms duration. The remaining cells (114), which could not be driven by habenula stimulation, exhibited spontaneous activity with a firing frequency from less than 1 Hz to greater than 100 Hz with a median firing frequency of 10 Hz. These experiments clearly demonstrate a direct habenulo-pineal fiber pathway and furthermore show that there are neuronal elements in the pineal which are only activated by habenula stimulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237549
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  • 6
    Electronic Resource
    Electronic Resource
    Morphological features of physiologically identified hypothalamic neurons as revealed by intracellular marking (1979)
    Springer
    Experimental brain research 34 (1979), S. 107-116 
    Details
    ISSN: 1432-1106
    Keywords: Parvocellular hypothalamic neurons ; Neurosecretion ; In vitro preparation ; Procion yellow ; Intracellular
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In the in vitro slice preparation intracellular recordings and injections of procion yellow (PY) were made in neurons of the hypothalamus. Of these neurons, one medial preoptic-anterior hypothalamus (MPO-AH) and four arcuate-ventromedial hypothalamus (ARC-VM) neurons were driven by electrical stimulation of the median eminence area (ME). Two other MPO-AH and five other ARC-VM neurons were driven by stimulation of the stria terminalis (ST). On the basis of the PY injections two morphologically distinct cell types were delineated: a larger multipolar cell type with a polygonal perikaryon was found with equal frequency in the MPO-AH and the ARC-VM. A smaller fusiform cell type was encountered mainly in the ARC-VM. On the secondary dendrites of both cell types spine-like appendages were seldom seen, but dendritic swellings were common. Some of the dendrites projected to capillaries in both areas and presumably contacted them. The axons were usually tortuous and could only be traced a short distance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00238344
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  • 7
    Electronic Resource
    Electronic Resource
    Hyperpolarization of hypothalamic parvocellular neurons by 17 β-estradiol and their identification through intracellular staining with procion yellow (1980)
    Springer
    Experimental brain research 40 (1980), S. 440-447 
    Details
    ISSN: 1432-1106
    Keywords: Parvocellular neurons ; Intracellular recordings ; Procion yellow ; Estrogen ; Humoral control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Intracellular recordings and injections of procion yellow (PY) were made in parvocellular neurons in hypothalamic slices of female guinea pigs. Eighty-five neurons, with an average resting membrane potential of -35 mV, were recorded in the arcuate (ARC) ventromedial (VM), and in the cellpoor zones between the ARC and VM. Eleven of the ARC neurons and four neurons from the cell-poor zone could be driven antidromically by median eminence (ME) stimulation, nine other neurons from the three areas could be driven orthodromically by stria terminalis (ST) stimulation. Twenty-eight parvocellular neurons were tested with 17 β-estradiol (E2), which was applied in the bathing medium as the free steroid. Eleven neurons (nine ARC and two cell-poor-zone neurons) were hyperpolarized 2 to 24 mV by 10−10 M E2 concentrations. 10−8 M estrone concentration was without effect on three of these cells. Through the intracellular injection of PY, the estrogen-sensitive neurons (N = 11) were identified as small fusiform cells with few dendrites. Spine-like appendages were found on only one of these cells. None of the larger pyramidal-like neurons of these areas responded to the application of E2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236152
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  • 8
    Electronic Resource
    Electronic Resource
    Immunocytochemical localization of luteinizing hormone-releasing hormone in neurons in the medial basal hypothalamus of the female rat (1982)
    Springer
    Experimental brain research 48 (1982), S. 97-106 
    Details
    ISSN: 1432-1106
    Keywords: Hypothalamic slice ; Immunocyto-chemistry ; Luteinizing hormone-releasing hormone ; Medial basal hypothalamus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Luteinizing hormone-releasing hormone (LHRH)-neurons were localized with a LHRH antiserum (WP-1) in the medial basal hypothalamus (MBH) of the female rat using the sagittal hypothalamic slice preparation in combination with the peroxidase-antiperoxidase immunocytochemical technique of Sternberger (1979). Numerous darklystained perikarya were visualized in the cell-poor zone, lateral arcuate nucleus and the median eminence. The processes of these neurons contributed to the intensely-stained fiber bundle above the tubero-infundibular sulcus. The fibers in this tract run in a rostrocaudal plane in the lateral external zone of the median eminence. Also, numerous fibers course into the internal zone of the median eminence, perpendicular to the rostrocaudal plane. Several LHRH-immunoreactive perikarya also were identified in the periventricular area caudal to the optic chiasm (retrochiasmatic area). The LHRH neurons were small (10 μm) bipolar neurons with fibers (dendrites) exiting from either pole which showed very little branching. It appears that the sagittal slice in combination with the appropriate fixation procedure and LHRH antiserum enabled us to demonstrate the presence of LHRH-immunoreactive perikarya in the MBH of the female rat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00239576
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  • 9
    Electronic Resource
    Electronic Resource
    Fulminant ulcerative colitis and parturition: a need to alter current management? (1994)
    Oxford, UK : Blackwell Publishing Ltd
    BJOG 101 (1994), S. 0 
    Details
    ISSN: 1471-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-0528.1994.tb13088.x
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  • 10
    Electronic Resource
    Electronic Resource
    Photoluminescence and passivation of silicon nanostructures (1994)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 65 (1994), S. 2386-2388 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: A new method was used to fabricate nanometer-scale structures in Si for photoluminescence studies. Helium ions were implanted to form a dense subsurface layer of small cavities (1–16 nm diameter). Implanted specimens subjected to annealing in a variety of atmospheres yielded no detectable photoluminescence. However, implantation combined with electrochemical anodization produced a substantial blueshift relative to anodization alone. This blueshift is consistent with the quantum confinement model of photoluminescence in porous silicon.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.113036
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