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  • 1
    Electronic Resource
    Electronic Resource
    The effects of pantothenic acid, cysteine and dithiothreitol in intact, reperfused pig hearts (1991)
    Springer
    Molecular and cellular biochemistry 105 (1991), S. 27-35 
    Details
    ISSN: 1573-4919
    Keywords: myocardial stunning ; amphiphiles ; free and short-chain CoA and carnitine ; free-radical scavengers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The objective of this study was to augment myocardial tissue levels of amphiphiles using a treatment protocol of pantothenic acid, cysteine and dithiothreitol (DTT) in 24hr fasted pigs and to test their influence on mechanical recovery in reperfusion. Eighteen pig hearts were extracorporeally perfused aerobically, subjected to regionally reversible ischemia in the left anterior descending perfusion system and reperfused. Nine hearts served as a placebo group; nine hearts were treated. All hearts received trace-labeled palmitate to measure fatty acid oxidation and were perfused with an infusion of 20% Intralipid to augment perfusate levels of fatty acids. Fasting alone in the presence of carbon substrates in the coronary perfusate was not sufficient to de-inhibit pantothenic acid kinase such that CoA synthesis was not enhanced. Tissue contents of triacylglycerols and phospholipids in reperfused myocardium were no different than in aerobic heart muscle but free CoA and free and total carnitine were reduced, suggesting a leakage of cytosolic contents across injured sarcolemma. Treatment significantly impaired mechanical recovery during reflow, presumable due to the noxious properties of DTT whose reported effects in heart muscle are wide ranging, difficult to predict in intact hearts and may be harmful.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00230372
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  • 2
    Electronic Resource
    Electronic Resource
    Alanine, glutamate, and ammonia exchanges in acutely ischemic swine myocardium (1992)
    Springer
    Basic research in cardiology 87 (1992), S. 184-192 
    Details
    ISSN: 1435-1803
    Keywords: Anoxia ; glycolysis ; heart ; ischemia ; myocardialmetabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Coronary artery disease causes an increase in glutamate uptake and alanine output by the heart. We assessed the effects of acute myocardial ischemia on alanine and glutamate exchange and ammonia production in 10 anesthetized open-chest domestic swine (46.9±0.7 kg). Coronary blood flow was controlled through an extracorporal perfusion circuit. After a nonischemic control period (aerobic) the blood flow in the left anterior descending coronary artery was reduced by 60%. Arterial and anterior interventricular venous samples where drawn before and during 35 min of ischemia. Subendocardial blood flow, measured using radiolabeled microspheres, decreased from 1.27±0.16 to 0.25±0.09 (ml/g)/min, and left-ventricular wall-thickening fell to 47% of aerobic values. Ischemia resulted in a significant increase in the rate of glucose uptake (p〈0.05) and a switch to net lactate production (p〈0.01). Ischemia did not affect the rates of alanine output (−0.9±1.0 vs. −0.3±0.3 μmol/min) or glutamate uptake (−0.4±1.1 vs. 0.3±0.6 μmol/min), but did increase the venous-arterial difference for ammonia (−4.1±4.1 to 52.7±5.5 μM, p〈0.0001) and the ammonia output (−0.33±0.24 to 1.34±0.14 μmol/min, p〈0.0001). In conclusion, acute ischemia did not stimulate greater alanine output or glutamate uptake. However, acute ischemia did cause an increase in anaerobic glycolysis rate and ammonia output, which reflects a profound disruption in myocardial energy metabolism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00801965
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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