ISSN:
1572-994X
Schlagwort(e):
HPV16
;
enhancer
;
cell type- specificity
;
Jun-B
;
c-Fos
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Medizin
Notizen:
Abstract The epithelial cell type- specific enhancer of the human papillomavirus (HPV) type 16 termed the long control region (LCR) carries three AP-1 binding sites. We investigated the roles of the AP-1 sites for transactivation of the LCR byJun-B that may be a cell type specific- transactivator for the HPVs in human fibroblasts in which expression of the endogenousjun-B gene is low. Transient expression ofJun-B alone poorly activated transcriptional activity of the LCR. However, when combined with c-Fos,Jun-B activated the LCR. The promoter-proximal AP-1 site was required for transactivation of the LCR byJun-B:c-Fos, but the site itself was not sufficient for the maximal response. The proposed cell type specific- regulatory element that harbors binding sites for NF1 as well as TEF-1 and PEA3 motifs, but neither other AP-1 sites nor the proximal enhancer region, could augment the transcriptional response of the promoter-proximal AP-1 site toJun-B:c-Fos.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00576977
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