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  • 1
    ISSN: 1573-482X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Capacitors with two kinds of lower electrodes were fabricated and their effects evaluated on the electrical characteristics of oxide–nitride–oxide (ONO) film. One of the electrodes was made of amorphous silicon film chemically deposited using a gas mixture of Si2H6–PH3; the other was made of poly-Si film deposited by SiH4 decomposition and doped by As+ ion implantation. The ONO thin dielectric layer was composed of natural oxide, CVD silicon nitride and thermal oxide formed on the silicon nitride. The capacitance, the leakage current, the dielectric breakdown field and the time-dependent dielectric breakdown (TDDB) were tested to evaluate the electrical properties of the capacitors. The leakage current and the dielectric breakdown voltage showed similar values between the two capacitors, whereas the TDDB under negative bias showed a great difference. This indicates that, with respect to electrical properties, the integrity of the oxide grown on the in situ P-doped amorphous silicon is better than the oxide grown on the As+ ion-implanted poly-Si. What is more, phosphorus in the amorphous silicon did not lead to any problems with junction depth, even after post heat treatment at 950°C.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 34 (1991), S. 726-729 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A hybrid bioreactor was developed for the production of secondary metabolites from high density cultivation of plant cell suspensions. Some of the advantages of both air-lift and cell-lift by agitation were combined. The addition of a decanting column also made it possble to run a perfusion system for high density culture or to run a two-stage culture efficiently. Cell growth and the production of berberine from Thalictrum rugosum in the hybrid bioreactor are reported in this paper. A cell density up to 31 g/l was obtained by perfusion without any problems in mixing or loss of cell viability and the specific berberine productivity was comparable to that in shake flasks. The maximum berberine concentration was 88 mg/l at 3 weeks operation and declined thereafter.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 35 (1991), S. 46-50 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A recombinant Saccharomyces cerevisiae producing hepatitis B surface antigen (HBsAg) exhibited growth-assciated product formation. By controlling the medium feed rate, based on the calculated amount of medium required for 1 h, a constant specific growth rate was obtained in the range of 1.12-0.18 h−1. In order to prolong the exponential growth phase, the medium feed rate was increased exponentially. A fedbatch cultivation method based on the production kinetics of batch culture enhanced HBsAg production ten times more than in batch culture. The reason for the increase can be explained by the fact that the production of HBsAg is expressed as an exponential function of time when the specific growth rate is controlled to a constant value in growth-associated product fromation kinetics. In the scale-up of this culture to 91, the specific growth rate could also be maintained constant and the HBsAg production trend was similar to that in a 1-l culture. However, ethanol accumulation occurred at a late stage in fed-bach culture. Ethanol produced was not reutilized and inhibited further cell growth.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Methyl jasmonate increased taxane production in suspension cultures of Taxus baccata Pendula. Time course changes of taxane production after methyl jasmonate addition were different from normal kinetics without elicitation. Baccatin III and 10-deacetyl baccatin III were detected first and paclitaxel, 10-deacetyl taxol and cephalomanine followed each other in sequence. Paclitaxel was not a dead-end metabolite.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Social psychology of education 1 (1996), S. 235-267 
    ISSN: 1573-1928
    Source: Springer Online Journal Archives 1860-2000
    Topics: Education , Psychology
    Notes: Abstract Data from observations of 232 elementary classrooms and from student questionnaires were used to test a model linking teacher classroom practices to students' sense of the classroom as a community (assessed by questionnaire) through intermediate effects on students' classroom behavior. The model was generally confirmed and showed that teacher practices (emphasis on prosocial values, elicitation of student thinking and expression of ideas, encouragement of cooperation, warmth and supportiveness, andreduced use of extrinsic control) were related to student classroom behaviors (engagement, influence, andpositive behavior), which, in turn, were related to students'sense of community. Teachers' encouragement of cooperative activities appeared to be particularly important in this sequence. The appropriateness of the model was tested for schools serving populations that were both high and low in level of poverty, and all estimates of path coefficients were found to be invariant across these groups.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A serum-free medium was constructed for the suspension culture of mammalian cells. Effects of the serum-free medium on the cultivation of T-lymphoblastoid cells secreting human immunodeficiency virus (HIV) were observed. The cell growth and HIV production in the serum-free medium were comparable to those in the medium supplemented with 10% fetal bovine serum (FBS), which showed a possibility of economical industrial application.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Suspension cells of Taxus brevifolia were cultured as an alternative source of taxol and related taxanes. The effects of basal medium, sugar type, and sugar concentration on growth and taxane production were investigated. To induce taxol production, modification of medium composition was necessary. Fructose supported the best taxol yield and the use of high concentration of sugar was desirable. The maximum level of taxol obtained after 10 days of culture in an optimized condition was 1.43 mg/L, which was 0.013% as a specific content.
    Type of Medium: Electronic Resource
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