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  • 1
    Electronic Resource
    Electronic Resource
    Comparative study of different hydrophobic devices for sampling lipid surface films and adherent microorganisms (1979)
    Springer
    Marine biology 53 (1979), S. 21-25 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Some simple devices for collection of lipid films and adhering microorganisms have been tested at various places along the Swedish west coast and in model systems. Information is given about enrichment of microorganisms and the magnitude of accumulation in these films. The present work indicates that the use of a Teflon sheet is a suitable method for lipid film sampling, both for microbiological and chemical purposes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00386525
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  • 2
    Electronic Resource
    Electronic Resource
    Distribution of lipolytic, proteolytic, and amylolytic marine bacteria between the lipid film and the subsurface water (1976)
    Springer
    Marine biology 39 (1976), S. 103-109 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The biochemical activity of marine bacteria adhering to the lipid film at the airwater interface was compared to that of bacteria inhabiting the subsurface water. The bacteria were isolated from the two levels at 7 stations, all located along Brofjorden on the Swedish West Coast. The isolates were tested for their ability to attack lipids, proteins, and carbohydrates. A higher percentage of active isolates was found in samples from the subsurface water than from the surface film. The accumulation of marine bacteria at the air-water interface is not due to their biochemical activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00395599
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  • 3
    Electronic Resource
    Electronic Resource
    Bacterial scavenging: Utilization of fatty acids localized at a solid-liquid interface (1982)
    Springer
    Archives of microbiology 133 (1982), S. 257-260 
    Details
    ISSN: 1432-072X
    Keywords: Bacterial scavenging ; Surface localized nutrients ; Leptospira ; Serratia ; Hydrophobic interactions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A model oligotrophic aquatic system involving localization of fatty acids on a solid surface was used to quantitate scavenging by three bacteria; Leptospira biflexa patoc 1 which adheres reversibly, pigmented Serratia marcescens EF190 which adheres irreversibly, and a non-pigmented hydrophilic mutant of EF190. The Leptospira and pigmented Serratia displayed two distinct scavenging strategies which are related to their different methods of adhesion. The Leptospira efficiently scavenged [1-14C] stearic acid from the surface in 24 h, whereas the pigmented hydrophobic Serratia initially showed a faster rate of removal but the overall rate was considerably slower than that of the Leptospira. The hydrophilic, non-pigmented Serratia required 50h incubation to remove significant amounts of the labelled fatty acid. The greater scavenging ability of the hydrophobic pigmented Serratia strain compared to the hydrophilic non-pigmented mutant could not be attributed to differences in viability of fatty acid metabolism. The hydrophobicity of the pigmented Serratia allows for firmer adhesion and greater interaction with the surface localized nutrients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00521286
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  • 4
    Electronic Resource
    Electronic Resource
    QUANTITATIVE ANALYSIS OF BACTERIAL HYDROPHOBICITY STUDIED BY THE BINDING OF DODECANOIC ACID (1980)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 7 (1980), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.1980.tb01572.x
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  • 5
    Electronic Resource
    Electronic Resource
    Interaction of pigmented wildtype and pigmentless mutant of Serratia marcescens with lipid surface film (1979)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 6 (1979), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1979.tb04293.x
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  • 6
    Electronic Resource
    Electronic Resource
    The Transient Phase Between Growth and Nongrowth of Heterotrophic Bacteria, with Emphasis on the Marine Environment (1987)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Microbiology 41 (1987), S. 25-49 
    Details
    ISSN: 0066-4227
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.mi.41.100187.000325
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  • 7
    Electronic Resource
    Electronic Resource
    Colonization in the fish intestinal tract and production of inhibitory substances in intestinal mucus and faecal extracts by Carnobacterium sp. strain K1 (1997)
    Oxford, UK : Blackwell Science Ltd
    Journal of fish diseases 20 (1997), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: A non-virulent Carnobacterium sp., designated strain K1, isolated from the gastrointestinal tract of Atlantic salmon, Salmo salar L., which produced inhibitory substances against bacterial fish pathogens, was examined in vitro for characteristics important for the colonization of the fish gastrointestinal tract and in vivo for persistence in the tract after oral dosing. In vitro growth experiments showed that the cells of this strain were metabolically active in both the intestinal mucus and faeces from salmonids. The production of growth inhibitors against the two common fish pathogens Vibrio anguillarum and Aeromonas salmonicida by Carnobacterium sp. strain K1 was demonstrated in vitro in mucus and faecal extracts. Furthermore, it was demonstrated that the Carnobacterium cells remained viable in the gastrointestinal tract for several days and that no detrimental effect to the fish was observed as a result of the presence of the bacterium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2761.1997.00316.x
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  • 8
    Electronic Resource
    Electronic Resource
    Is the turbot, Scophthalmus maximus (L.), intestine a portal of entry for the fish pathogen Vibrio anguillarum? (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 19 (1996), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The role of the intestinal tract in Vibrio anguillarum infection of turbot, Scophthalmus maximus (L.), fingerlings was investigated in two in vivo models and the possible mechanisms involved were studied in vitro. Viable V. anguillarum cells were detected in spleens from more than 50% of the fish administered the pathogen orally or rectally, suggesting that the intestinal tract is a portal of entry for V anguillarum. In transmission electron micrographs, V. anguillarum- like cells were seen close to the rectal epithelium, suggesting penetration of the mucus layer, but no epithelial cell penetration or endocytosis was evident. Attachment to intact turbot intestines was investigated, and 80% or more of the bacterial cells still remained attached after serial washings. A significantly higher number of cells attached to rectal segments than to the other intestinal segments. In vitro, V. anguillarum cells did not adhere specifically to intestinal mucus, but rather accumulated close to intestinal mucus interfaces and subsequently penetrated them. It is proposed that the intestinal ttact of tutbot is a portal of entry for V. anguillarum and that the cells penetrate the intestinal mucus overlaying the epithelial cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2761.1996.tb00129.x
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  • 9
    Electronic Resource
    Electronic Resource
    Starvation of marine flounder, squid and laboratory mice and its effect on the intestinal microbiota (1986)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 38 (1986), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Starvation processes of microorganisms in natural ecosystems were studied, both in vivo and in vitro, using marine animals (flounder and squid) and laboratory mice. In flounder, starvation resulted in the mixed intestinal microbiota maintaining its viability but decreasing its cell volume. It was also observed that during starvation there was an increased liability for adhesion of th microbiota to both the oil-water interface in the hexadecane water separation technique, and to Sepharose beads with either exposed hydrophobic or cationic charge groups. The population also exhibited the capacity to respond immediately to the addition of nutrients. When the flounder microbiota was starved in vitro the ratio of bacteria cultured on high nutrient: low nutrient media decreased with time of starvation. A similar effect on the intestinal microbiota of squid was observed in vivo when the animals were starved. The in vivo starvation of the mouse also produced a decrease in the mean bacterial cell volume which was concurrrent with a promotion of coliform bacteria. A coliform isolate exhibited similar starvation survival characteristics in vitro. From the data obtained from the flounder, squid and mice, it was concluded that components of the large intestinal microbiota exhibited the starvation survival characteristics previously reported for laboratory studies of planktonic bacteria, when exposed to energy- or nutrient-limited conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1986.tb01728.x
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  • 10
    Electronic Resource
    Electronic Resource
    Vibrio vulnificus: a physiological and genetic approach to the viable but nonculturable response (2000)
    Springer
    Journal of infection and chemotherapy 6 (2000), S. 115-120 
    Details
    ISSN: 1437-7780
    Keywords: Key words VBNC ; Vibrio vulnificus ; Adaptive response ; Starvation ; Resuscitation ; Marine bacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In this review, we focus on studies of the viable but nonculturable response (VBNC) of Vibrio vulnificus, a significant and aggressive human pathogen, as a model system for the general understanding of the VBNC response. This response is characterized physiologically as the inability to culture an organism on media that normally supports its growth, and yet those cells retain indicators of metabolic activity. Implicit in this definition is that it may be possible to return or resuscitate VBNC cells to active division on laboratory media. Since its original description in 1985, the VBNC response has been recognized in a range of bacteria. Study of the VBNC response has traditionally focused on physiological methods aimed at demonstrating that VBNC cells are indeed viable but have a specific block that prevents them from dividing on laboratory media, and such study has attempted to identify conditions that unequivocally demonstrate the resuscitation of VBNC cells. With the advent of molecular genetics, VBNC studies have begun to focus on genetics as a means to determine whether there are specific genes or regulatory pathways responsible for the development of the VBNC response. Thus, by combining information from physiological and genetic experiments, it is hoped that it can be determined whether the VBNC response represents a genetically programmed physiological adaptation similar to sporulation and outgrowth or whether VBNC represents the slow loss of function on the way to cellular death.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00012150
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