ISSN:
1432-2048
Keywords:
Cell culture
;
Cinnamoyl putrescines
;
Nicottana
;
Phenolics
;
Phosphate
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The inorganic phosphate of the liquid nutrient medium was completely taken up by freshly inoculated cells of Nicotiana tabacum L. within the first 2 d of culture. Thus intracellular ortho-phosphate concentrations of approx. 0.06 M were accumulated, which upon growth of the cultures were diluted by cell division and subsequent cell growth. Cells from different stages of the growth cycle containing progressively decreasing levels of phosphate were transferred to a phosphate-free medium which normally stimulates the formation of cinnamoyl putrescines. The resulting accumulation of these compounds was inversely correlated with the intracellular phosphate level, whereas a direct linear relationship in the phosphate concentration was found with further growth in the phosphate-free medium.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00385545
Permalink