ISSN:
1432-0428
Keywords:
Insulin resistance
;
lipolysis
;
free fatty acids
;
glycerol
;
glucose clamp technique
;
glucose production
;
glucose utilization
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Although extensive evidence indicates that free fatty acids can decrease glucose utilization in vitro, it is still controversial how an increase in lipolysis affects glucose metabolism in man. To test the hypothesis that an increase in lipolysis is related to insulin resistance, we examined the effect of lipid-heparin infusion on glucose metabolism in ten normal subjects by the euglycaemic glucose clamp technique and isotopic determination of glucose turnover. In the control euglycaemic clamp studies with insulin infusion at 0.2 and 1.0 mU·kg−1·min−1, endogenous glucose production was suppressed from the basal rate of 2.0±0.3 mg· kg−1min−1 to 1.1±0.7 mg·kg−1·min−1 and -0.4±0.7mg· kg−1min−1 respectively. Glucose utilization increased from the basal rate of 2.0±0.3 mg·kg−1min−1 to 2.3±0.5mg· kg−1min−1 and 5.9±1.8 mg·kg−1min−1 respectively. When the euglycaemic clamp studies were coupled with lipid-heparin infusion at comparable low and high rates of insulin infusion, endogenous glucose production increased (1.8± 0.7 mg·kg−1·min−1, p〈0.001, and 0.3±0.6 mg·kg−1· min−1, p〈0.05, respectively), and glucose utilization decreased (2.1±0.3 mg·kg−1·min−1, not significant, and 3.2±0.7 mg·kg−1·min−1, p〈0.001 respectively). These data suggest that the artificial induction of intravascular lipolysis by lipid-heparin infusion leads to a state of insulin resistance in man.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00277409
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