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  • 1
    Electronic Resource
    Electronic Resource
    Kinetics of Catalytic Transfer Hydrogenation of Soybean Oil in Microwave and Thermal Field (1994)
    s.l. : American Chemical Society
    The @journal of organic chemistry 59 (1994), S. 7433-7436 
    Details
    ISSN: 1520-6904
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/jo00103a041
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    On-line measurement and analysis of yeast flocculation (1997)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 53 (1997), S. 179-184 
    Details
    ISSN: 0006-3592
    Keywords: flocculation ; on-line measurement ; floc formation rate ; floc dispersion rate ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The ability of yeast to flocculate is important in different separation processes, especially in the beer industry. Because of the regulation purposes, there is a need for online monitoring. With the presented measuring set-up, consisting of a peristaltic pump, a photometer, and a computer, it is possible to determine the onset of flocculation as well as to follow flocculation intensity and the concentration of nonflocculated cells. It was found that for the yeast strain Saccharomyces cerevisiae ZIM 198 the decrease of nonflocculated cells (after flocculation has occurred) during the exponential growth can be described by an exponential equation for the first-order process, whereas the increase of free cells due to dispersion of the flocs during the stationary phase follows the form of the growth curve. It was also demonstrated that the absorbency profiles of yeast sedimentation can be described by the second-order equation suggested by Stradford and Keenan for the decrease of cell concentration during sedimentation. © 1997 John Wiley & Sons, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 3
    Electronic Resource
    Electronic Resource
    Nonflocculent versus total biomass ratio as a criterion for starting the biomass separation process (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 59 (1998), S. 647-650 
    Details
    ISSN: 0006-3592
    Keywords: biomass separation ; flocculation ; biomass measurement ; yeast ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: We introduce the ratio of nonflocculent versus total biomass as a criterion for starting cell separation from the medium. This criterion can be applied for the automation of the process regardless of the process dynamics. Its minimum indicates the optimum period of time for the start of the separation process with regard not only to nonflocculent cell concentration, but also medium attributes. In contrast to the concentration of nonflocculent cells, which has two minima, first at the beginning of the process and another broader one in the period during which maximum flocculation is present, the ratio has a single minimum and can therefore be implemented as a criterion for cell separation. To calculate the ratio value, in addition to an on-line method for nonflocculent biomass measurement described elsewhere, an on-line method for the total biomass of flocculent yeast is proposed. It is based on the absorbency measurement of the cell biomass, previously deflocculated by EDTA. Therefore, it can be applied in bioprocesses with transparent media and yeast that can be deflocculated by EDTA. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 59:647-650, 1998.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 4
    Electronic Resource
    Electronic Resource
    Dynamic Capacity Studies of CIM (Convective Interaction Media)® Monolithic Columns (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 39-43 
    Details
    ISSN: 0935-6304
    Keywords: Monoliths ; CIM® monolithic column ; dynamic binding capacity ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The characterization of CIM® DEAE monolithic columns in terms of dynamic binding capacity is presented in this paper. Breakthrough experiments were performed for capacity determination. Bovine serum albumin (BSA) was used as a model protein. It is shown that CIM® monolithic columns have good batch-to-batch reproducibility as well as long-term stability. The experiments performed under different linear velocities demonstrated that the dynamic capacity is unaffected at least up to a linear velocity of 2450 cm/h. Furthermore, the breakthrough curve slope is constant, indicating that the capacity would remain constant at even higher linear velocities. The adsorption isotherm of BSA dissolved in 20 mM Tris-HCl buffer shows a constant capacity of around 30 mg/mL of support down to a concentration of 20 μg/mL. The capacity is substantially influenced by the ionic strength; however, 20% of the maximal capacity is still preserved at 0.3 M NaCl.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
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