ISSN:
1432-0584
Keywords:
Erythrocytes
;
G-6-PD Deficiency
;
Screening
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Red blood cell lysates were applied to DEAE cellulose paper for detection of glucose-6-phosphate dehydrogenase. Glucose-6-phosphate dehydrogenase (G-6-PD) was separated from 6-phosphogluconate dehydrogenase (6-PGD) by DEAE-cellulose paper-chromatography and their activities were detected on the chromatogram by applying the reaction mixture. The fluorescence of NADPH formed by G-6-PD was not interfered with by 6-PGD activity. This is a sensitive procedure which detects slight loss of G-6-PD in red blood cells and could be applied to the detection of G-6-PD deficiency.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00320496
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