ISSN:
0003-276X
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
Notes:
3β-Hydroxysteroid dehydrogenase activity was localized in ovaries removed from rats during normal pseudopregnancy. Ovarian sections were incubated in media containing either pregnenolone (P-one) or dehydroepiandrosterone (DHA). P-one-3β-HSD activity appeared strongest on day 6 whereas DHA-3β-HSD was highest on day 13. NADH2 diaphorase activity was localized and observed to be greater than 3β-HSD.Both P-one- and DHA-3β-HSD activities were localized in primary-, secondary- and tertiary follicles; granulosa and theca interna cells have a variable potential for steroidogenesis. Mature tertiary follicles appear most responsive of the follicular population in steroidogenic potential. In atretic follicles, theca interna cells showed a marked ability for potential steroid production and may be one of the more stable sites of steroidogenesis. Interstitial tissue is a major site for potential estrogenic and progestational biosynthesis during the functional life of the ovary. Luteal cells exhibited strong ability for DHA-3β-HSD activity during the ongoing process of pseudopregnancy while P-one-3β-HSD activity declined in these cells as pseudopregnancy progressed. In summary, at the onset of pseudopregnancy, it appears that ovarian P-one-3β-HSD activity is greater than DHA-3β-HSD activity. Near the termination of this condition, DHA-3β-HSD activity is greater than P-one-3β-HSD activity in the rat.
Additional Material:
9 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/ar.1091940209
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