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  • 1
    ISSN: 1432-0789
    Keywords: Phospholipids ; Microbial biomass ; Phosphatase ; Cropping systems ; Long-term experiments Prairie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In agricultural ecosystems that have had consistent cropping histories, standard microbial methods may be used to evaluate past and present practices. Our objective was to evaluate several microbial methods that best indicate cropping histories and soil quality on long-term plots. We selected soil microbial carbon (C), phospholipid analyses, direct counts of total fungal and bacterial biomass, and soil enzymes (phosphatases) to measure direct and indirect microbial activity on the Sanborn Field and Tucker Prairie. The Sanborn Field has been under various cropping and management practices since 1888 and the Tucker Prairie is an uncultivated site. Seven different plots were chosen on the Sanborn Field and random samples were taken in the summit area on the Tucker Prairie, which represented a reference site. Soil microbial biomass C, phospholipids, and enzyme activity were reflective of the cropping and management histories observed on the Sanborn Field. Enzymatic activity was highly correlated to soil organic matter. The direct counts of fungal and bacterial biomass showed that fungal populations dominated these soils, which may be attributed to soil pH. Soil microbial biomass C and enzyme assays seemed to be better potential indicators of cropping histories than the other methods tested in the long-term plots.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    World journal of microbiology and biotechnology 5 (1989), S. 95-104 
    ISSN: 1573-0972
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Description / Table of Contents: Résumé On a évalué plusieurs facteurs qui influencent la qualité physique et biologique d'inocula de légumes à base d'engrais. L'incorporation de 2.5, 5 ou 8% d'huile d'arachide ou d'huile de paraffine dans les granules d'engrais conduit à des granules stables et fluides, exempts d'agrégation. Toutefois, la composition chimique des engrais limite de façon significative la survie des Rhizobia. Ainsi, la roche phosphatée en granules (PR) a permis une meilleure survie que les engrais à respectivement 0/6.5/12.4 ou à 0/8.7/16.6 d'N, P et K. Les Rhizobia suspendus dans l'huile appliqués aux granules PR à 8% ont survécu de manière acceptable à 30°C mais se sont révélés moins tolérants à la température de 60°C bien que l'engrais contenant encore 103 à 104 cellules par gramme à cette température élevée après 56 jours de conservation. Les souches deRhizobium diffèrent également entre elles quant à leur tolérance vis-à-vis du type d'engrais et de la température élevée. Les résultats suggèrent que les inocula à base d'engrais peuvent constituer un inoculum important pour un usage particulier, notamment comme alternative aux inocula conventionnels dans plusieurs régions du monde.
    Notes: Summary Several factors affecting the physical and biological quality of fertilizer-based legume inocula were evaluated. Application of 2.5, 5 or 8% (v/w) peanut oil or paraffin oil in a fertilizer granule resulted in stable and flowable granules, free from aggregation. However, chemical composition of fertilizers significantly limited rhizobial survival in that granulated phosphate rock (PR) promoted survival better than either 0/6.5/12.4 or 0/8.7/16.6 (N/P/K) fertilizers. Oil-suspended rhizobia applied to granular PR at 8% survived acceptably at 30°C but was less tolerant at 60°C although the fertilizer still contained 103 to 104 cells/g at the elevated temperature after 56 days of storage.Rhizobium strains also differed significantly in tolerance to fertilizer type and elevated temperatures. The results suggest that fertilizer-base inocula can be an important special purpose inoculant for use as an alternative to conventional inocula in many areas of the world.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Bulletin of environmental contamination and toxicology 42 (1989), S. 676-681 
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Bulletin of environmental contamination and toxicology 43 (1989), S. 421-427 
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Bulletin of environmental contamination and toxicology 55 (1995), S. 689-696 
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 47 (1997), S. 279-287 
    ISSN: 1573-5044
    Keywords: bacteria ; biological control ; electron microscopy ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The interaction of two rhizobacterial isolates,Pseudomonas fluorescens isolate LS 102 andFlavobacterium balustinum isolate LS 105, with leafy spurge cells at the cellular level was studied using scanning and electron microscopy. Leafy spurge callus tissue inoculated with either isolate showed considerable changes compared to non-inoculated tissue. The attachment of rhizobacteria to cell surfaces was associated with the elaboration of fibrillar material which may anchor bacteria to surfaces and contribute to mediation of the phytotoxic effect caused by rhizobacteria. At the ultracellular level, inoculated callus tissue showed numerous cell alterations including esiculation and convolution of the plasmalemma, cell wall degradation and disorganization of the cytoplasm, similar to those detected in the whole plant. It is concluded that callus tissue may provide an excellent working model to investigate the mode and/or mechanism of action of potential biocontrol agents on their host plants.
    Type of Medium: Electronic Resource
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