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  • 1
    Electronic Resource
    Electronic Resource
    Ammonia-excreting mutants of Klebsiella pneumoniae with a pleiotropic defect in nitrogen metabolism (1997)
    Springer
    Archives of microbiology 166 (1997), S. 388-393 
    Details
    ISSN: 1432-072X
    Keywords: Key words Ammonium metabolism ; glnAntrBC ; Nitrogenase regulation ; Nitrogen control ; Ammonia ; excretion ; Amino acid utilization ; Ntr
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Enterobacterial mutants defective in the nitrogen control regulatory system (Ntr) generally display a pleiotropic phenotype with regard to expression and regulation of several enzymes and transport systems involved in the assimilation of N sources. This report describes the isolation and characterization of similar pleiotropic mutants of Klebsiella pneumoniae that cannot be complemented by ntr genes. The strains excreted ammonia, were unable to grow on a number of N sources, and contained low glutamine:2-oxoglutarate amino transferase and normal, but unmodifiable glutamine synthetase activities and a nitrogenase level largely unaffected by ammonium, but still repressible by an amino acid mixture. Genetic studies suggested that this phenotype is due to overexpression of an unknown regulatory protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01682984
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The gltF gene of Klebsiella pneumoniae: cloning and initial characterization (1991)
    Springer
    Molecular genetics and genomics 229 (1991), S. 479-482 
    Details
    ISSN: 1617-4623
    Keywords: Nitrogen control ; gltF gene ; Klebsiella pneumoniae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary From a gene bank of Klebsiella pneumoniae M5a1, a 1.7 kb gene fragment was isolated which was able to restore the Ntr+ phenotype and ammonium (methylammonium) transport, but not glutamate synthase in an Escherichia coli glt mutant (glutamate synthase deficiency). The fragment strongly hybridized with the gltF regulatory gene from E. coli. After subcloning the fragment into an overexpression vector, a protein with a molecular weight of 27000 dalton was identified as the gene product. The results indicate that the fragment cloned contains the gltF gene from K. pneumoniae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267472
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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