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  • 1
    Electronic Resource
    Electronic Resource
    Copenhagen : International Union of Crystallography (IUCr)
    Applied crystallography online 30 (1997), S. 739-743 
    ISSN: 1600-5767
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Geosciences , Physics
    Notes: The phase behaviour and phase transition of the tetradecyldimethylaminoxide micellar system TDMAO/ D2O have been investigated by small-angle neutron scattering (SANS) at temperatures T between 287 and 325 K, pressures up to P = 842 MPa and at TDMAO concentrations of 55 and 102 mmol l−1. A pressure-induced structural (shape) phase transition was observed for the whole pressure versus temperature range, with a slope of the transition line of dTm/dP ∼ 0.31° MPa−1. The reversibility and reproducibility of this phase change were tested. The SANS experiments showed a pressure-induced structural phase transition from a low-pressure phase (i), where rod-like micelles with a radius of R = 1.94 ± 0.03 nm were present, to a high-pressure phase (ii). An accurate determination of the structure of phase (ii) has not been possible up to now. In phase (ii), a discrete correlation peak in the Q range 1.8–2.2 nm−1 was found.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of plant growth regulation 14 (1995), S. 91-97 
    ISSN: 1435-8107
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Catecholamines (CAs) are neurotransmitters in mammals. They have been found in 44 plant families, but no essential metabolic function has been established for them. They are precursors of benzo[c]phenanthridine alkaloids, which are the active principal ingredients of many medicinal plant extracts. CAs have been implicated to have a possible protective role against insect predators, injuries, and nitrogen detoxification. They have been shown to promote plant tissue growth, somatic embryogenesis from in vitro cultures, and flowering. CAs inhibit indole-3-acetic acid oxidation and enhance ethylene biosynthesis. They have also been shown to enhance synergistically various effects of gibberellins.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell reports 14 (1995), S. 641-644 
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Epinephrine at 10–100 μM stimulated somatic embryogenesis from orchardgrass (Dactylis glomerata L.) leaves cultured on SH medium with 30 μM of indole-3-acetic acid (IAA). Ethylene emanation was increased at epinephrine concentrations greater than 10 μM. Decarboxylation by the leaves of [1-14C]IAA included in the medium was decreased almost 3-fold by 10 μM epinephrine. Epinephrine at 10 μM enhanced the number of regenerated plants on SH medium with 30 μM dicamba (SH-30). Ethylene emanation was increased by epinephrine concentrations of 500 μM and greater included in SH-30 but somatic embryogenesis was decreased. Addition of 8 μM CoCl2, 6H2O (an ethylene biosynthesis inhibitor) to medium with 500 μM epinephrine decreased ethylene emanation to the control level but did not alleviate the decreased embryogenic response.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Methods in cell science 15 (1993), S. 204-209 
    ISSN: 1573-0603
    Keywords: chrysanthemum ; experimental design ; incomplete block design ; orchardgrass ; somatic embryogenesis ; shoot organogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Most experiments in tissue culture employ either completely randomized or randomized complete block designs. The ability of orchardgrass (Dactylis glomerata L.) leaf halves and segments from individual leaves of chrysanthemum (Dendranthema grandiflora Tzvelev.) to respond similarly in vitro to identical treatments allows application of an alternative experimental design based on blocking by leaf. Incomplete block designs conserve experimental materials and reduce labor while maintaining the statistical power and efficiency of more conventional designs. They also allow combination with nesting (hierarchal designs) to monitor different sources of variability or with factorial studies. Application of incomplete block designs for orchardgrass (somatic embryogenesis) and chrysanthemum (shoot organogenesis) leaf cultures are presented.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Biotechnology techniques 8 (1994), S. 467-472 
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The objective of this study was to develop a system to maintain orchardgrass (Dactylis glomerata L.) leaf segments in contact with solid medium in petri dishes during different orientation of the dishes. To ensure contact with the medium, leaf segments were overlaid with 1800 μm Teflon™ mesh. This was secured with a polypropylene ring which fitted between the petri dish lid and the mesh. This procedure did not affect the somatic embryogenesis response. A significant difference (P=0.05) in increased ethylene accumulation from overlaid segments was recorded on day 5.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-5087
    Keywords: aminooxyacetic acid ; Dactylis glomerata ; ethylene ; low temperature ; somatic embryogenesis ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The objectives of this study were to determine the effects of low temperature (4 °C) preincubation on somatic embryogenesis from orchardgrass (Dactylis glomerata L.) leaf cultures and to relate these effects to ethylene emanation during the preincubation and incubation periods. Experiments were also conducted with an ethylene biosynthesis inhibitor aminooxyacetic acid (AOA). Segments from the innermost two leaves were cultured on SH medium with 30 μM dicamba at 4 °C for 1 to 7 d before transfer to 21 °C. Results from a paired design showed that the embryogenic response of leaf segments preincubated at 4 °C was equal or superior to nonpreincubated leaves at all time periods. Ethylene emanation was decreased during the low temperature incubation. Transfer of leaf segments from 4 °C to 21 °C was accompanied by a burst of ethylene which rose to control levels within 30 min. AOA at 20 and 40 μM decreased ethylene emanation but did not stimulate the embryogenic response. We conclude that the stimulation of somatic embryogenesis by low temperature is probably due to factors other than suppression of ethylene biosynthesis.
    Type of Medium: Electronic Resource
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