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  • 1
    Electronic Resource
    Electronic Resource
    Folding in vitro and transport in vivo of pre-β-lactamase are SecB independent (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 5 (1991), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The rate of folding of the precursor of β-lactamase is not influenced by the presence of SecB under conditions in which GroEL/ES retards the folding. Wild-type β-lactamase and several mutants in the signal or the mature protein, affecting either transport or enzyme kinetics and probably folding, were examined for total expression, total enzymatic activity, and transported β-lactamase (in vivo resistance) in secB- and secB+ strains. We conclude that there is no indication of any relevant interaction between SecB and pre-β-lactamase in vitro, nor did the secB- mutation affect the transport of wild–type β-lactamase or any of the mutants in vivo. Thus, putative Escherichia coli‘folding modulators’must be of limited specificity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1991.tb01832.x
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Stable transformation and regulated expression of an inducible reporter construct in Candida albicans using restriction enzyme-mediated integration (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 75-80 
    Details
    ISSN: 1617-4623
    Keywords: Key words Candida albicans URA3 ; Restriction enzyme-mediated integration ; Transformation ; MAL2
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  To allow the regulated expression of cloned genes in Candida albicans, a plasmid was constructed using the inducible promoter of the C. albicans MAL2 gene. To demonstrate that the MAL2 promoter could regulate cloned genes placed under its control, a fusion construct was made with the coding sequence of the C. albicans URA3 gene. This plasmid was introduced into a Ura- strain of C. albicans using the process of restriction enzyme-mediated integration (REMI). This procedure involves the transformation of the BamHI-linearized plasmid in the presence of BamHI enzyme. The majority of transformants generated contained insertions of the plasmid at chromosomal BamHI sites. All transformants examined were inducible for URA3 expression, which was determined by growth analysis and by measuring the level of URA3 gene product activity. The Ura+ phenotype of the transformants was stable during growth under nonselective conditions. This system offers the advantages of stable transformation, easy recovery of integrated DNA, and inducible expression of genes in C. albicans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02174347
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Stable transformation and regulated expression of an inducible reporter construct inCandida albicans using restriction enzyme-mediated integration (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 75-80 
    Details
    ISSN: 1617-4623
    Keywords: Candida albicans URA3 ; Restriction enzyme-mediated integration ; Transformation ; MAL2
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To allow the regulated expression of cloned genes inCandida albicans, a plasmid was constructed using the inducible promoter of theC. albicans MAL2 gene. To demonstrate that theMAL2 promoter could regulate cloned genes placed under its control, a fusion construct was made with the coding sequence of theC. albicans URA3 gene. This plasmid was introduced into a Ura− strain ofC. albicans using the process of restriction enzyme-mediated integration (REMI). This procedure involves the transformation of theBamHI-linearized plasmid in the presence ofBamHI enzyme. The majority of transformants generated contained insertions of the plasmid at chromosomalBamHI sites. All transformants examined were inducible forURA3 expression, which was determined by growth analysis and by measuring the level ofURA3 gene product activity. The Ura+ phenotype of the transformants was stable during growth under nonselective conditions. This system offers the advantages of stable transformation, easy recovery of integrated DNA, and inducible expression of genes inC. albicans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02174347
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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