Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Differentiation of human lymphocytes by pokeweed mitogen in vitro: Studies in normal and in immunodeficient subjects (1977)
    Springer
    Journal of molecular medicine 55 (1977), S. 743-749 
    Details
    ISSN: 1432-1440
    Keywords: Immunglobulinsynthese ; Pflanzenlektine ; Primäre Immunmangelsyndrome ; Suppressor Lymphozyten ; Immunoglobulin synthesis ; Plant lectins ; Primary immunodeficiencies ; Suppressor lymphocytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Immunoglobulin (Ig) synthesis and secretion by peripheral blood lymphocytes activated by pokeweed mitogen (PWM), and by phytohemagglutinin (PHA) was measured in normal individuals and in subjects with primary immunodeficiency. Unstimulated cultures demonstrated stable, low Ig synthesis of all major Ig classes; PWM showed a peak of Ig synthesis at day 5; PHA cultures demonstrated a late peak of Ig production at day 9. Three cases of common variable immunodeficiency showed different patterns of data when the percentage of B cells in blood and Ig production in vitro were used as parameters. Two persons with immunodeficiency and thymoma showed decreased Ig production in vitro, and had suppressor cells capable of blocking Ig production by normal lymphocytes in co-cultivation experiments.
    Notes: Zusammenfassung Immunglobulinsynthese und Sekretion der Lymphozyten von Normalpersonen und Patienten mit primären Immundefekten wurde nach Stimulierung mit Pokeweed Mitogen oder Phytohaemagglutinin gemessen. Unstimulierte Kulturen zeigten eine stabile, geringe Immunglobulinsynthese aller drei Immunglobulinklassen. Immunglobulinsynthese in Pokeweed Mitogen stimulierten Kulturen zeigte einen Gipfel an Tag 5, phytohaemagglutinin-stimulierte Kulturen zeigten einen Gipfel an Tag 9. Drei Fälle von variablem Immunmangelsyndrom zeigten unterschiedliche Muster, wenn der Prozentsatz von B Lymphozyten und die in vitro Immunglobulinsynthese als Parameter herangezogen wurden. Zwei Patienten mit Immunmangelsyndrom bei Thymom zeigten verminderte Immunglobulinsynthese in vitro und hatten Suppressorzellen, die in Kokultivierungsexperimenten die Immunglobulinsynthese von Normallymphozyten blockierten.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01476961
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Genetic Marker of the γA2 Subgroup of γA Immunoglobulins (1969)
    [s.l.] : Nature Publishing Group
    Nature 223 (1969), S. 1247-1248 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] A genetic marker for the γA2 immunoglobulin has been delineated by precipitating and haemagglutination inhibition techniques with a human antiserum. The marker varies widely in incidence in different population groups. Although associated with all the major Gm gene complexes, a close linkage ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/2231247a0
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Characteristics of Suppressor Cell Activity Appearing in Cocultures of Two Individuals with Immunodeficiency with Thymoma (1980)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 11 (1980), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Suppressor cell activity in two individuals (S1 and S2) with immunodeficiency with thymoma (ID-THY) was studied in peripheral blood mononuclear cells in pokeweed mitogen-stimulated single culture and cocultures. Secreted Ig was measured by radioincorporation and immunoprecipitation after 5-7 days. Control cocultures of normal/normal cells showed, in most cases, a percentage observed to expected ratio (% O/E) of cpm Ig near 100%. However, augmentation (% of O/E 〉 150) was often encountered, whereas suppression (% O/E 〈50) was found only ran In forty-two cocultures. In ID-THY/normal cell cocultures the degree of suppression by ID-THY cells varied widely when the same or different cocultivanls were retested. This finding could be explained in part an inverse correlation between the amount of secreted Ig produced by normal cells in single culture and the degree of suppression of the same normal by ID-THY cells in cocultures. A panel of normal cells were all suppressed when a range of S1 or S2/normal cell ratios were tested, weighing against genetic differences in suppressibility in the above system. ID-THY cells failed to block Ig secretion of human lymphoblastoid line cells, suggesting that the mechanism of suppression was related to a block in differentiation rather than interference mith Ig synthesis per se. An experiment using cocultivants separated by a Millipore membrane showed that suppression was mediated by a humoral factor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1980.tb00203.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    IgD and the Fetal Immune Response (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 6 (1977), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: IgD was detected in 84% of cord sera at term by an inhibition-of-hemagglutination test, in contrast to low incidences reported earlier. Cord blood IgD and maternal serum IgD values were unrelated to each other, consistent with a fetal origin of the IgD. The cord blood IgD failed to correlate, either directly or inversely, with cord blood IgA or IgM, suggesting that its presence does not reflect fetal synthesis of specific antibody but rather may be developmental.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1977.tb02156.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Human IgD and IgAl Compete for D-Galactose-Related Binding Sites on the Lectin Jacalin (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 26 (1987), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Lectin selectivity for human ig classes is based on carbohydrate differences. Earlier reports that the lectin jacalin precipitated human igA were confirmed and supplemented by the current study, which demonstrates that jacalin also binds human IgD as evaluated by micro-ELlSA and SDS-PAGE. Experimental findings indicated that: (i) Monoclonal and polyclonal (sera) IgD. IgAl, bul not IgA2, IgM, or IgGI-4 reacted with jacalin, (ii) Six tested monoclonal IgD proteins each bound approximately equally to jacalin when antigenicity rather than protein concentration was measured; the results weigh against the presence of jacalin-delectable IgD subclasses or genetic variants, (iii) IgD and IgAl both associated maximally in 4-8h at 4°C. There was no dissociation at 4°C but limited dissociation occurred at 37°C after 24h, (iv) Both IgD and IgAl were eluted from jacalin by galaetose-related sugars, (v) IgD and IgAI bind competitively to jacalin. The results suggested that jacalin reacts with O-linkcd oligosaccharide N-acetyl-galactosamine (GalN) residues found on the hinge region of both IgD and IgAl, Jacatin also interacted with one major and several minor unidentified sera proteins. The findings offer an approach to the isolation of serum polyclonal IgD and to the characterization of the unusual carbohydrates of the human delta heavy chain with respect to their function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1987.tb02256.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Change in Ig Expression of a Human Lymphoblastoid Line During Long-Term Culture (1976)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 5 (1976), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A human lymphoblastoid line, in continuous culture for over 3 years, showed changes in Ig expression with time. The following pattern of events was suggested by studies of Ig synthesis and secretion performed at intervals. In a mass culture containing a heterogeneous cell population, Ig expression may shift because of selection acting on the different phenotypes. In human lymphoblastoid lines there may be a tendency for selection to favor IgMx-producing cells. In clones, including those that synthesize multiple H chains, substantial determinism for Ig expression is present.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1976.tb03028.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Induction of immunoglobulin synthesis in corticosteroid-treated blood lymphocytes of a patient with acquired agammaglobulinemia (1981)
    Springer
    Journal of clinical immunology 1 (1981), S. 94-100 
    Details
    ISSN: 1573-2592
    Keywords: Immunodeficiency ; corticosteroids ; Ig synthesisin vitro
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Coculture experiments between lymphocytes of a 17-year-old immunodeficient male, DL, and a group of normal subjects, assaying pokeweed mitogen (PWM)-stimulated Ig secretion as a measure of B-cell function, revealed immunoregulatory abnormalities. Initial studies disclosed that DL had corticosteroid-sensitive T suppressor (Ts) cells capable of blocking Ig secretion by both HLA-identical and HLA-nonidentical cells in coculture. Cocultures of DL's peripheral blood mononuclear cells could be induced to secrete Ig in large amounts after certain maneuvers—the most informative of which involved mixing prednisolone-treated DL mononuclear cells with any normal T lymphocytes except those from DL himself. When these same experimental manipulations were performed individually, i.e., prednisolone treatment of cultured DL cells to remove Ts activity, or mixing equal numbers of normal T cells with untreated DL mononuclear cells, Ig was not produced. The data indicated that the T-cell abnormalities in DL included an excess of Ts cells and a deficiency of T helper (Th) cells. When repeat studies were performed later in the clinical course, during which interval a number of clinical interventions were attempted, it was found that the patient's cells were no longer corticosteroid sensitive and, further, they suppressed only HLA-identical cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00915385
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...