ISSN:
1573-4838
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
,
Technology
Notes:
Abstract Phospholipase A2 (PLA2) hydrolyses certain phospholipids of low density lipoproteins. PLA2-treated LDL is known to be rapidly cleared from plasma. A prototype plasma filter containing immobilizedCrotalus atrox PLA2 on agarose beads was developed. After a 90 min treatment with the extracorporeal device, plasma cholesterol concentration in cholesterol-fed NZW rabbits decreased by 32%. The decrease was dependent on the enzymatic activity in the plasma filter. The decrease in plasma cholesterol of hypercholesterolemic rabbits that were treated with control reactors (agarose beads only) was 5%. White and red blood cell counts and platelets remained unchanged during the treatment. Plasma cholesterol reduction (25–40%) was also obtained following intravenous injection of active PLA2 to modify plasma lipoproteins. PLA2 infusion created a radical change in biliary composition. Bile phospholipid composition was 90–95% lysophosphatidylcholine as compared with more than 95% before injection of active PLA2. Phospholipid and bile salts total mass increased by 10%. While biliary secretion rate of protein increased by 10%, biliary secretion rate of cholesterol remained unchanged. This technique is specific for lipoproteins, does not require any fluid replacement of sorbent regeneration, and offers a potential new approach for lowering serum cholesterol and LDL levels.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00702937
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