ISSN:
0935-6304
Keywords:
Supercritical Fluid Extraction (SFE)
;
Gas chromatography
;
Dicofol
;
Fish
;
LC50
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Toxicity evaluation of Dicofol to Astyanax bimaculatus schubarti, a characteristic fish species living in tropical rivers and lakes was carried out through LC50 - 96 Hours. These experiments were performed under laboratory controlled conditions with atmospheric air flow and dilution water at 25°C in the static mode, supercritical fluid extraction (SFE) with pure CO2 and CO2 modified with hexane and methanol were used at 50, 70, 80, and 100°C and 300 atm. Several collection modes were studied to extract Dicofol from fish samples. The extraction efficiencies were directly comparedd with those obtained after 8 h of Soxhlet extraction using the same clean-up with Florisil and analysis by HRGC/ECD and HRGC/MS as a confirmatory analytical technique. The SFE recoveries at temperatures lower than 80°C were typically lower than soxhlet recoveries; however a temperature increase enhanced the efficiency of SFE. The results showed that under certain conditions, supercritical fluid gave higher extractio power (extracted 11 % more pesticide), shorter extraction time, and lower solvent consumption than Soxhlet, thus affording an excellent alternative to the conventional method for extracting Dicofol from fish sample.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240200705
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