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Feedback-stabilized focal plane control for light microscopes (1993)

Lanni, Frederick

[S.l.] : American Institute of Physics (AIP)

Review of Scientific Instruments 64 (1993), S. 1474-1477

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ISSN: 1089-7623

Source: AIP Digital Archive

Topics: Physics , Electrical Engineering, Measurement and Control Technology

Notes: The study of the three-dimensional structure of biological specimens by light microscopy requires accurate and precise control of focal plane setting. For imaging of live cells, speed is also an important constraint, due to Brownian and active motions within the specimen. We have used a linear capacitance gauge and piezoelectric elements in a closed-loop configuration to stabilize the specimen-to-objective lens distance in a microscope with a precision of 0.012 μm. The circuit also allows for rapid, arbitrary, electronic control of focal plane setting independent of the mechanical focusing drive of the microscope.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1063/1.1144066

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Enhancement of axial resolution in fluorescence microscopy by standing-wave excitation (1993)

Bailey, Brent ; Farkas, Daniel L. ; Taylor, D. Lansing ; [et al.]

[s.l.] : Nature Publishing Group

Nature 366 (1993), S. 44-48

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ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] Three-dimensional imaging in a microscope requires both high transverse and axial resolution. The well-known Rayleigh formula sets transverse resolution at 0.2 um by direct imaging. In contrast, axial resolution of compact object features in fluorescence optical sectioning microscopy (FOSM) ...