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Novel mutations affecting leaf stearate content and plant size in Arabidopsis (1997)

Lightner, J. ; Lark, Ellen ; James, D. ; [et al.]

Springer

Theoretical and applied genetics 94 (1997), S. 975-981

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ISSN: 1432-2242

Keywords: Key words Arabidopsis ; Fatty acid ; Suppressor ; Development ; Mutant

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The fab2-1 mutant of Arabidopsis is an extreme dwarf as a direct result of an increase in the levels of stearate (18 : 0) in membrane lipids. We isolated a series of lines in which second-site suppressor mutations partly alleviate the dwarf phenotype. In all four of the suppressor lines examined, restoration of more normal morphology is accompanied by decreases in leaf 18 : 0 content. Three of the isolated suppressors suppress the high stearate phenotype in both leaves and seeds. The effects of one of the suppressors, TW2-1, is limited to the leaves. A second allele at the fab2 locus, fab2-2, was also identified and plants homozygous for this allele where intermediate in both plant size and 18 : 0 content between wild-type Arabidopsis and fab2-1 mutants. The alleles at fab2 and the suppressor mutations provided a total of nine genotypes which were analyzed to demonstrate a clear-cut relationship between leaf 18 : 0 content (0.7–19.6% of total leaf fatty acids) and reductions in plant size (24–4 mm). These results illustrate the utility of suppressor analysis for addressing problems in biochemistry and plant biology. They also indicate that the genetic control of plant lipid composition is more complex than previously appreciated.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s001220050504

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Axonemes paralyzed by the presence of dyneins unable to use ribose-modified ATP (1994)

Lark, Ellen ; Omoto, Charlotte K.

New York, NY : Wiley-Blackwell

Cell Motility and the Cytoskeleton 27 (1994), S. 161-168

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ISSN: 0886-1544

Keywords: fluorescent nucleotide analogs ; methylanthraniloyl ATP ; anthraniloyl ATP ; Chlamydomonas ; axonemal mutants ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: Substrate analogs are useful for studying the structures of active sites and for distinguishing between similar enzyme activities. Fluorescent ribose-modified ATP analogs were used to investigate the functional differences between dynein ATPases. These analogs reactivate (support the movement of) sea urchin sperm axonemes, yet they do not reactivate wild-type Chalmydomonas axonemes. Surprisingly, the analogs reactivate the axonemes of mutants completely missing the outer arm dyneins. Competition experiments using ATP and these analogs provide strong evidence that the analogs bind to all dynein active sites but fail to release a subset of dyneins from rigor. We suggest that this subset of Chlamydomonas outer arm dyneins unable to use the analogs remains in rigor in the presence of the analogs and paralyzes the axoneme. © 1994 Wiley-Liss, Inc.

Additional Material: 4 Ill.