ISSN:
0003-276X
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
Notes:
The mandibular incisor of the 16 day embryonic mouse was divided into three visibly equal thirds; each third was explanted in vitro to test its capabilities for independent development. Ameloblasts and odontoblasts, present in the distal third at the time of isolation, fail to continue their differentiation. This third flattens, and the tissues become disorganized. Grouping several distal thirds in a single culture did not promote tissue differentiation. The embryonic proximal third remains cohesive The tissues differentiate; both ameloblasts and odontoblasts deposit extracellular matrix. Approximately half of the cultures of the intermediate third show differentiated odontoblasts and extracellular matrix after 11 culture days. Measurements of the volume changes of ameloblasts, odontoblasts, and general mesenchyme were taken over the first six culture days for each third of the rudiment. The volume changes recorded, and the counts of mitotic figures reflect the developmental behavior of each fraction of the rudiment.
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/ar.1091660309
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