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Rhythmic histone acetylation underlies transcription in the mammalian circadian clock (2003)

Etchegaray, Jean-Pierre ; Lee, Choogon ; Wade, Paul A. ; [et al.]

[s.l.] : Macmillian Magazines Ltd.

Nature 421 (2003), S. 177-182

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ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] In the mouse circadian clock, a transcriptional feedback loop is at the centre of the clockwork mechanism. Clock and Bmal1 are essential transcription factors that drive the expression of three period genes (Per1–3) and two cryptochrome genes (Cry1 and Cry2). The Cry proteins feedback to ...

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1038/nature01314

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Light does not degrade the constitutively expressed BMAL1 protein in the mouse suprachiasmatic nucleus (2003)

Von Gall, Charlotte ; Noton, Elizabeth ; Lee, Choogon ; [et al.]

Oxford, UK : Blackwell Science, Ltd

European journal of neuroscience 18 (2003), S. 0

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ISSN: 1460-9568

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: Biological rhythms in mammals are driven by a central circadian clock located in the suprachiasmatic nucleus (SCN). At the molecular level the biological clock is based on the rhythmic expression of clock genes. Two basic helix–loop–helix (bHLH)/PAS-containing transcription factors, CLOCK and BMAL1 (MOP3), provide the basic drive to the system by activating transcription of negative regulators through E box enhancer elements. A critical feature of circadian timing is the ability of the clockwork to be entrained to the environmental light/dark cycle. The light-resetting mechanism of the mammalian circadian clock is poorly understood. Light-induced phase shifts are correlated with the induction of the clock genes mPer1 and mPer2 and a subsequent increase in mPER1 protein levels. It has previously been suggested that rapid degradation of BMAL1 protein in the rat SCN is part of the resetting mechanism of the central pacemaker. Our study shows that BMAL1 and CLOCK proteins are continuously expressed at high levels in the mouse SCN, supporting the hypothesis that rhythmic negative feedback plays the major role in rhythm generation in the mammalian pacemaker. Using both immunocytochemistry and immunoblot analysis, our studies demonstrate that BMAL1 protein in the mouse SCN is not affected by a phase-resetting light pulse. These results indicate that rapid degradation of BMAL1 protein is not a consistent feature of resetting mechanisms in rodents.