ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
To improve ostegenic healing efficiency by demineralized bone matrix, we evaluatedthe ectopic bone formation induced by variously demineralized allogenic cortical bone matricesat subcutaneous and muscular sites in rats. The rat tubular cortical bone matrices weredemineralized in heated 0.6N HCl at 60 °C for 5 and 20 mins, respectively, using a controlledheatultrasonic cleaner and implanted in rat dorsal subcutaneous pouches and thigh muscles for1-3 weeks. The influence of the demineralized condition of bone matrix on cellular proliferationand osteogenic differentiation was also evaluated in vitro by MTT assay and ALP staining. Thecortical matrices were completely demineralized within 20 mins by sonication and heating ofdiluted 0.6 N HCl. The sonicated bone matrices in heated acidic solution at 60 °C revealed noadverse immunogenic and inflammatory response in vivo regardless of demineralized condition.Cellular proliferation and osteoblastic differentiation was facilitated by more fullydemineralized. Ectopic bone formation was induced only by demineralized bone matrices andwere more favorable in fully demineralized matrices. The ectopic bone induction was morefavorably in subcutaneous pouches than in muscular tissue. These findings suggest that a fullydemineralized cortical bone matrix maximizes osteogenic repair by exposing more bioactivemolecules which in turn induce chondro- and osteognic differentiation of mesenchymal cellsaround the implanted matrices, and that the sonication of diluted 0.6 N HCl heated at 60 ° C is arapid and effective method for sterile demineralized graft preparation
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/54/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.342-343.105.pdf
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