ISSN:
1573-6776
Keywords:
Alkyl-O-glycosides
;
β-galactosidase
;
β-glucosidase
;
microwave irradiation
;
reverse hydrolysis
;
transglycosylation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract 2-(4-Methoxybenzyl)-1-cyclohexyl-β-d-glucopyra nosides (1b and 2b) and 2-(4-methoxybenzyl)-1-cyclohexyl-β-d-galactopy ranosides (1c and 2c), models for glycosidic juvenogens, were synthesized using either D-glucose or D-galactose [in their natural form (3 and 5) or activated form (4 and 6)], and the respective racemic cis or trans isomers of 2-(4-methoxybenzyl)-1-cyclohexanol (1a and 2a) by either enzymic reverse hydrolysis or transglycosylation under both standard heating and microwave irradiation. Commercially available almond β-glucosidase (EC 3.2.1.21) or β-galactosidase (EC 3.2.1.23) from Escherichia coli were employed using different acetonitrile/water mixtures [9/1 (v/v) for the reverse hydrolysis, and 4/1 (v/v) for the transglycosylation].
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1005539626471
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