Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Expression of exogenous DNA in rat liver cells after liposome-mediated transfection in vivo
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 174 (1991), S. 1223-1231 
    Details
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0006-291X(91)91552-N
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Functional analysis of DNA-elements involved in transcriptional control of the human glucose transporter 2 (GLUT 2) gene in the insulin-producing cell line βTC-3 (1995)
    Springer
    Diabetologia 38 (1995), S. 112-115 
    Details
    ISSN: 1432-0428
    Keywords: Gene expression regulation ; insulinoma ; glucose transporter ; transcription ; promoter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The uptake of glucose into pancreatic beta cells as a ‘non-rate-limiting-step’ is guaranteed by the expression and action of the high-Km glucose transporter 2 (GLUT 2). This transporter is not saturated by physiological plasma glucose levels and hence functions as a “glucose sensor/glucoreceptor”. Here we describe DNA-elements of the human GLUT 2 gene promoter which contribute to transcriptional control in the insulin-producing cell line βTC-3. Nested 5′-as well as 3′-deletions of a DNA-fragment containing up to 1245 bp of the 5′-flanking region and up to 308 bp of the first exon of the human GLUT 2 gene were investigated for their ability to control the expression of a CAT reporter gene in βTC-3 cells. For tissue-specific transcriptional control 5′-deletional analysis revealed that the region −220/+309 was sufficient. Truncation from the 3′-end from nucleotide +308 to +204 led to a threefold drop in CAT expression.In vitro DNase I footprinting analysis was performed to delineatecis-elements within the region −220/+1. Five specifically protected areas could be defined. [Diabetologia (1995) 38: 112–115]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02369360
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Functional analysis of DNA-elements involved in transcriptional control of the human glucose transporter 2 (GLUT 2) gene in the insulin-producing cell line βTC-3 (1995)
    Springer
    Diabetologia 38 (1995), S. 112-115 
    Details
    ISSN: 1432-0428
    Keywords: Key words Gene expression regulation ; insulinoma ; glucose transporter ; transcription ; promoter.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The uptake of glucose into pancreatic beta cells as a ’non-rate-limiting-step' is guaranteed by the expression and action of the high-Km glucose transporter 2 (GLUT 2). This transporter is not saturated by physiological plasma glucose levels and hence functions as a “glucose sensor/glucoreceptor”. Here we describe DNA-elements of the human GLUT 2 gene promoter which contribute to transcriptional control in the insulin-producing cell line βTC-3. Nested 5′- as well as 3′-deletions of a DNA-fragment containing up to 1245 bp of the 5′-flanking region and up to 308 bp of the first exon of the human GLUT 2 gene were investigated for their ability to control the expression of a CAT reporter gene in βTC-3 cells. For tissue-specific transcriptional control 5′-deletional analysis revealed that the region −220/+309 was sufficient. Truncation from the 3′-end from nucleotide +308 to +204 led to a threefold drop in CAT expression. In vitro DNase I footprinting analysis was performed to delineate cis -elements within the region −220/+1. Five specifically protected areas could be defined. [Diabetologia (1995) 38: 112–115]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001250050260
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...