ISSN:
1662-9752
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Creatures have evolved extremely intelligent and complex adaptive systemsfor conducting their movements. They are protein motors with typical sizes of a fewtens of nanometers. Protein motors include three major protein families, myosin, kinesinand dynein, which participate in a wide range of cellular processes, using energy fromthe hydrolysis of adenosinetriphosphate ATP. To harness these protein motors to powernanometer-scale devices, we have investigated effective and non-destructive methodsfor immobilizing protein motors on surfaces and to arrange the output of these motors,e.g. force and movement, to be in a defined direction. We found NEB-22 to be usefulfor retaining the abilities of protein motors to support the movement of protein filaments.We fabricated various patterns of tracks of NEB-22 on coverslips and protein motorswere introduced and immobilized on glass surface. The trajectories of protein polymerswere confined to these tracks. Simple patterns readily biased and guide polymermovement confining it to be unidirectional. In addition, having used dynein c purifiedfrom Chlamydomonas flagellar axoneme, we showed that microtubules driven bysurface-bound dynein were self-organized into dynamic streams through collisionsbetween the microtubules and their subsequent joining
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/02/15/transtech_doi~10.4028%252Fwww.scientific.net%252FMSF.539-543.3290.pdf
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