ISSN:
1432-1203
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary There is a synergistic effect between hydroxyurea (HU) and high concentrations of thymidine (TdR) on the induction of the common fragile sites at 3p14 and 16q23. The frequencies of expression of these fragile sites were significantly higher than in the controls when excessive TdR (0.5mM) and HU (4mM) were added into lymphocyte cultures in MEM medium 24h and 2h before harvest. Two conditions for the maximum expression of common fragile sites, such as fra(3)(p14), fra(16)(q23) are discussed. The TdR-HU fra(3)(p14)-inducing system might be modified effectively and consistently to improve the prenatal diagnosis of the fragile X syndrome.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00273656
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