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  • 1
    Electronic Resource
    Electronic Resource
    Modified Airy function method for modeling of direct tunneling current in metal–oxide–semiconductor structures (2001)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 79 (2001), S. 1831-1833 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Using a modified Airy function (MAF) to solve the Schrödinger equation in the whole metal–oxide–semiconductor structure, a fully quantum-mechanical model of direct tunneling current from an inverted p-Si substrate through ultrathin oxides is presented. The effects of tunneling on the electrostatic potential and the distribution of electrons are also included when self-consistently solving the Schrödinger and Poisson equations in silicon. Due to the semianalytical nature of the MAF method, the model has high efficiency. Model results are compared with experimental data and show excellent agreement. Moreover, an approximately linear relationship between the logarithm of the direct tunneling current and oxide thickness is found out. © 2001 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1403658
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  • 2
    Electronic Resource
    Electronic Resource
    Integration of chemical-genetic and genetic interaction data links bioactive compounds to cellular target pathways (2004)
    [s.l.] : Nature Publishing Group
    Nature biotechnology 22 (2004), S. 62-69 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Bioactive compounds can be valuable research tools and drug leads, but it is often difficult to identify their mechanism of action or cellular target. Here we investigate the potential for integration of chemical-genetic and genetic interaction data to reveal information about the pathways and ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt919
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  • 3
    Electronic Resource
    Electronic Resource
    The synthetic genetic interaction spectrum of essential genes (2005)
    [s.l.] : Nature Publishing Group
    Nature genetics 37 (2005), S. 1147-1152 
    Details
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] The nature of synthetic genetic interactions involving essential genes (those required for viability) has not been previously examined in a broad and unbiased manner. We crossed yeast strains carrying promoter-replacement alleles for more than half of all essential yeast genes to a panel of 30 ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/ng1640
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  • 4
    Electronic Resource
    Electronic Resource
    Stochastic analysis of a maintainable robot-safety system with common-cause failures (2004)
    Bingley : Emerald
    Journal of quality in maintenance engineering 10 (2004), S. 136-147 
    Details
    ISSN: 1355-2511
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Technology , Economics
    Notes: This paper presents reliability and availability analyses of a robot-safety system having one robot and n-redundant safety units with common-cause failures. The system failure rates and the partially failed system repair rates are assumed constant, and the failed system repair time is assumed arbitrarily distributed. Markov and the supplementary variable methods were used to perform mathematical analysis of this model. Generalized expressions for state probabilities, system availabilities, reliability, mean time to failure, and variance of time to failure are developed. Some plots of these expressions are shown.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1108/13552510410539204
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  • 5
    Electronic Resource
    Electronic Resource
    Efficient plant regeneration from rice protoplasts in general medium (1990)
    Springer
    Plant cell reports 9 (1990), S. 216-220 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We established an efficient and reproducible procedure for protoplast propagation and fertile plant regeneration of rice (Oryza sativa L.) cultivars Nipponbare and Taipei 309. Selection of scutellum-derived secondary calli, the use of General medium and nurse culture were all found to be critical in the procedure. When 5 basal media (Murashige and Skoog, RY-2, modified R2, Amino Acid and General media) were compared, suspension callus growth rate, protoplast yield and plating efficiency were all about 30% higher in General medium than in the second-best R2 medium. Only one month was required to develop suspension cultures for protoplast isolation using General medium. A plating efficiency as high as 17% and a plant regeneration frequency of 67% were achieved by the improved procedure. Agronomic traits of protoplast- and seed-derived plants were found to be similar.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232183
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  • 6
    Electronic Resource
    Electronic Resource
    Production of fertile transgenic peanut (Arachis hypogaea L.) plants using Agrobacterium tumefaciens (1996)
    Springer
    Plant cell reports 15 (1996), S. 653-657 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fertile transgenic plants of peanut (Arachis hypogaea L. cv. New Mexico Valencia A) were produced using an Agrobacterium-mediated transformation system. Leaf section explants were inoculated with A. tumefaciens strain EHA105 harboring the binary vector pBI121 containing the genes for β-glucuronidase (GUS) and neomycin phosphotransferase II (NPTII). Approximately 10% of the shoots regenerated on selection medium were GUS-positive. Five independent transformation events resulted in the production of 52 fertile transgenic peanut plants. On average, 240 d were required between seed germination for explant preparation and the production of mature t1 seed by T0 plants. Molecular analysis of transgenic plants confirmed the stable integration of the transgenes into the peanut genome. GUS expression segregated in a 3∶1 Mendelian ratio in most T1 generation plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231918
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  • 7
    Electronic Resource
    Electronic Resource
    Production of fertile transgenic peanut (Arachis hypogaea L.) plants using Agrobacterium tumefaciens (1996)
    Springer
    Plant cell reports 15 (1996), S. 653-657 
    Details
    ISSN: 1432-203X
    Keywords: Abbreviations: GUS, β-glucuronidase; NPTII, neomycin-phosphotransferase II; MS medium, Murashige and Skoog medium (1962); BA, N6-benzyladenine, NAA, 1-naphthaleneacetic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Fertile transgenic plants of peanut (Arachis hypogaea L. cv. New Mexico Valencia A) were produced using an Agrobacterium-mediated transformation system. Leaf section explants were inoculated with A. tumefaciens strain EHA105 harboring the binary vector pBI121 containing the genes for β-glucuronidase (GUS) and neomycin phosphotransferase II (NPTII). Approximately 10% of the shoots regenerated on selection medium were GUS-positive. Five independent transformation events resulted in the production of 52 fertile transgenic peanut plants. On average, 240 d were required between seed germination for explant preparation and the production of mature T1 seed by T0 plants. Molecular analysis of transgenic plants confirmed the stable integration of the transgenes into the peanut genome. GUS expression segregated in a 3:1 Mendelian ratio in most T1 generation plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050092
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  • 8
    Electronic Resource
    Electronic Resource
    In-vitro selection of Vitis vinifera `Chardonnay' with Elsinoe ampelina culture filtrate is accompanied by fungal resistance and enhanced secretion of chitinase (2000)
    Springer
    Planta 211 (2000), S. 200-208 
    Details
    ISSN: 1432-2048
    Keywords: Key words: Anthracnose ; Disease resistance (grapevine) ; Proembryogenic mass ; Vitis (disease resistance)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract.  Proembryogenic masses of grapevine (Vitis vinifera L.) `Chardonnay' (clone 02Ch) were exposed to the culture filtrate of Elsinoe ampelina (deBary) Shear, the causal agent of anthracnose disease. After four or five cycles of recurrent in-vitro selection with medium containing 40% fungal culture filtrate, putative resistant lines RC 1 and RC 2 respectively, were established. The selected lines inhibited the growth of E. ampelina and Fusarium oxysporium (Schlecht.) (isolated from watermelon) in a dual-culture assay and reduced the growth of mycelium on a conditioned-medium test, thus suggesting the involvement of extracellular compounds in resistance. Sodium dodecyl sulfate-polyacrylamide (SDS-PAGE) gel electrophoresis of extracellular proteins from spent suspension-culture medium showed enhanced secretion of new proteins by selected lines. A 36-kDa protein was immunodetected by a chitinase antiserum. This chitinase continued to express constitutively in differentiated somatic embryos and also in the intercellular fluids of plants regenerated from the selected lines. Somatic embryos from selected lines grew uninhibitedly in a medium containing 40% fungal culture filtrate, whereas non-selected (control) somatic embryos became necrotic and died within a few days. Plants regenerated from selected lines exhibited resistance to infection by E. ampelina in both greenhouse tests and detached leaf bioassays. Results suggest that embryogenic cells can be selected for resistance following in-vitro selection, resulting in resistant plants. Whether or not resistant cells pre-existed in the original embryogenic culture or were induced by the selection pressure could not be determined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004250000285
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  • 9
    Electronic Resource
    Electronic Resource
    Engineered resistance to tomato spotted wilt virus in transgenic peanut expressing the viral nucleocapsid gene (1997)
    Springer
    Transgenic research 6 (1997), S. 297-305 
    Details
    ISSN: 1573-9368
    Keywords: peanut ; engineered virusresistance ; tomato spotted wiltvirus ; transformation ; nucleocapsid ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The nucleocapsid gene of tomato spotted wilt virus Hawaiian L isolate in a sense orientation, and the GUS and NPTII marker genes, were introduced into peanut (Arachis hypogaea cv. New Mexico Valencia A) using Agrobacterium-mediated transformation. Modifications to a previously defined transformation protocol reduced the time required for production of transformed peanut plants. Transgenes were stably integrated into the peanut genome and transmitted to progeny. RNA expression and production of nucleocapsid protein in transgenic peanut were observed. Progeny of transgenic peanut plants expressing the nucleocapsid gene showed a 10- to 15-day delay in symptom development after mechanical inoculations with the donor isolate of tomato spotted wilt virus. All transgenic plants were protected from systemic tomato spotted wilt virus infection. Inoculated non-transformed control plants and plants transformed with a gene cassette not containing the nucleocapsid gene became systemically infected and displayed typical tomato spotted wilt virus symptoms. These results demonstrate that protection against tomato spotted wilt virus can be achieved in transgenic peanut plants by expression of the sense RNA of the tomato spotted wilt virus nucleocapsid gene
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018462729127
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  • 10
    Electronic Resource
    Electronic Resource
    Efficient plant regeneration from protoplasts of Arachis paraguariensis Chod. et Hassl. using a nurse culture method (1993)
    Springer
    Plant cell, tissue and organ culture 34 (1993), S. 83-90 
    Details
    ISSN: 1573-5044
    Keywords: Arachis species ; nurse culture ; plant regeneration ; protoplasts ; tissue culture ; wild peanut
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An efficient protocol has been developed for protoplast culture and plant regeneration from wild peanut (A. paraguariensis) using a nurse culture method. Protoplasts were isolated from suspension cultures initiated from leaf-derived callus, imbedded in agarose blocks and co-cultured with nurse cells of the same species. Up to 10% of the protoplasts divided and formed compact callus colonies. The protoplast plating efficiency was correlated with both the length of the nurse cell co-cultivation period and the protoplast plating density. The optimal nurse culture duration was 14 d. The optimal plating density was 2×104 protoplasts/ml plating medium. Multiple shoots (up to 10 shoots per colony) were readily regenerated from protoplast-derived callus after transfer of callus to semi-solid modified MS medium containing 0.5 mg l-1 NAA and 1 mg l-1 BA. Plantlets with normal leaflets were obtained by rooting shoots on porous rootcubes saturated with modified MS medium containing 1 mg l-1 NAA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00048467
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