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  • 1
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Four tropical marine fish cell lines have been established from the eye, fin, heart and swim bladder of grouper, Epinephelus awoara (Temminck & Schlegel). Optimum media and temperature conditions for maximum growth were standardized. The eye and swim bladder cells were mostly epithelial, but the fin and heart cells were mostly fibroblastic. The viability of cells was 95% after 1 year of storage in liquid nitrogen (−196 °C). Besides these four cell lines, previously established grouper brain, kidney and liver cell lines were also used for a viral susceptibility study which showed that all the cell lines were sensitive to grouper iridovirus, whereas only brain, fin and liver cell lines were susceptible to the yellow grouper nervous necrosis virus (a nodavirus). Electron microscopy studies of the grouper irido- and nodaviruses in ultrathin sections of infected cells showed an abundance of viral particles in the cytoplasm of the virus-infected cells indicating the effective replication of these two viruses. It is suggested that these cell lines can be used for the isolation of putative fish specific viruses and provide a valuable tool to study the mechanisms of host–pathogen interactions. Furthermore, these cell lines upon transfection, using pEGFP-C1 and pEGFP-aMT2.5 (ayu metallothionein promoter), produced significant fluorescent signals indicating their utility for exogenous studies.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 15 (1992), S. 0 
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Waxapple (Syzygium samarangense Men. et. Perry) plants receiving up to 40d of continuous flooding treatment showed no symptoms of physiological disorder, but the treatment resulted in early flowering. In this report, several physiological parameters of flooded plants are compared with those of nonflooded plants. Both control plants and 9-d-flooding-treated plants exhibited aerenchyma formation in the cortex tissue beginning 5 cm from the root tip. After 7d flooding treatment, the oxygen consumption rate of the root section was only 20% of that of the controls. Following flooding treatment, the roots showed an increase in alcohol dehydrogenase activity as well as an increase in three isozymes. However, malate dehydrogenase activity was decreased, and no significant change of NADP-malic enzyme activity was observed. There were no significant differences in levels of ethylene, 1-aminocyclopropane-1-carboxylic acid and 1-(malonylamino) cyclopropane-1-carboxylic acid in petiole and roots of flooded and non-flooded plants during the stage examined. It is inferred that the presence of aerenchyma in the root cortex allows a higher level of internal gas exchange, and thus, makes waxapple surprisingly flood tolerant. However, reduced root oxygen consumption rate may have limited root respiration rate and vegetative growth.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of the American Chemical Society 99 (1977), S. 953-954 
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of the American Chemical Society 94 (1972), S. 4342-4343 
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of the American Chemical Society 94 (1972), S. 4343-4345 
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Pure and applied geophysics 149 (1997), S. 667-688 
    ISSN: 1420-9136
    Keywords: Key words: 3-D velocity structure, circular ray tracing, San Andreas Fault, seismicity.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract —The three-dimensional P-wave velocity structure of the Bear Valley region of central California is determined by applying a circular ray-tracing technique to 1735 P-wave arrivals from 108 locally recorded earthquakes. Comparison of the results obtained from one-dimensional and laterally varying starting models shows that many of the features in the structure determined are fairly insensitive to the choice of the starting model. Velocities associated with the Gabilan granites southwest of the San Andreas Fault are slightly higher than those in the Franciscan formation to the northeast, and these two features are separated in the southern part of the region by a narrow fault zone with very low velocities. In the southeastern part of the region, where the Gabilan granites do not abut the San Andreas Fault, the low velocities of the fault zone cross over to the southwestern side of the fault. They also appear to extend to depths of at least 15km, thus locally reversing the contrast across the San Andreas Fault that prevails farther to the northwest. In the northwestern part of the region, the low velocities of the fault zone split and follow the surface traces of the San Andreas and Calaveras Faults, but do not appear to extend to depths much deeper than about 6km. There also appears to be a well-defined contrast in structure in the middle of the Santa Clara Valley, suggesting the existence of a fault in the basement of the valley that may be a southern extension of the Sargent Fault into this region. Relocated hypocenters beneath the San Andreas Fault cluster in a zone that dips about 80° southwest and intersects the surface trace of the fault in the southern part of region.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    European journal of pediatrics 156 (1997), S. 572-574 
    ISSN: 1432-1076
    Keywords: Key words Congenital diaphragmatic hernia ; Pulmonary hypoplasia ; Pancreas fibrosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Bilateral agenesis of the diaphragm is a rare, life-threatening malformation. Infants with this defect rarely survive to have surgical intervention. We report a 32-week premature female infant who was born to a 36-year-old mother via vaginal delivery. The pregnancy course was complicated by hypertension and polyhydramnios. Cytogenetic study showed a normal 46 XX female karyotype. She had cyanosis, respiratory distress and scaphoid abdomen at birth. A roentgenograph confirmed the diagnosis of diaphragmatic hernia. Surgery was performed at 21 h of age. Bilateral agenesis of diaphragm, herniation of abdominal organs and oesophagus and pulmonary hypoplasia were noted. Furthermore, stomach and spleen were adherent to the mediastinum and vertebrae. The patient developed hypotension and persistent hypoxaemia and expired at age of 26 h. Autopsy revealed bilateral agenesis of diaphragm, hypoplasia of lungs, and pancreas fibrosis with mild hypoplasia of islets of Langerhans. Conclusion Bilateral agenesis of diaphragm associated with pancreas fibrosis is a rare entity, and its clinical significance needs further investigation.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 159 (1989), S. 343-348 
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 160 (1989), S. 276-281 
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Tetrahedron: Asymmetry 5 (1994), S. 199-202 
    ISSN: 0957-4166
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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