ISSN:
0173-0835
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
We describe a system for accurate photometry and analysis of two-dimensional (2-D) gels, and its application to both high and low resolution gels. Image acquisition is video-based and includes methods of data correction necessary for accurate photometry. The analysis is based upon a method described previously by Lutin et al. [8], which we have modified for improved stability and adapted to run on our PDP 11/60 computer. We illustrate analysis of a typical gel with a sequence of images showing the data while Gaussian components are estimated, removed, added and fitted as the iterative algorithm proceeds. We conclude by demonstrating the use of a mathematical curve-of-growth model for calibrating peak volumes vs. protein loading.
Additional Material:
10 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150040111
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