ISSN:
1546-1696
Source:
Nature Archives 1869 - 2009
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
[Auszug] We replaced the wild-type TOX operon of Bordetella pertussis with in vitro mutated, detoxified alleles by electroporetic transformation using unmarked linear DNA. Uptake of DNA was selected by transient ampicillin resistance and two simultaneous recombination events resulted in gene-replacement at ...
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1038/nbt1190-1025
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