ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
The archaebacterial-type aspartyl-tRNA synthetase (AspRS2) from the thermophilic eubacterium Thermus thermophilus was crystallized using the hanging-drop vapour-diffusion method. Crystals grew at pH 9.5 in the presence of PEG 8000 and NaCl. A native diffraction data set has been collected at 2.5 Å resolution using synchrotron radiation and cryocooling. Crystals belong to the orthorhombic space group P212121, with unit-cell parameters a = 57.3, b = 121.9, c = 166.9 Å and VM = 3.03 Å3 Da−1. There is one dimer of Mr 96 000 per asymmetric unit. A molecular-replacement analysis gave solutions for the rotation and translation functions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444901009611
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