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  • 1
    Electronic Resource
    Electronic Resource
    Murine cytotoxic T-cell response to alphavirus is associated mainly withH- 2D k (1978)
    Springer
    Immunogenetics 7 (1978), S. 551-561 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A secondary in vitro response to alphaviruses Bebaru, Sindbis, and Semliki Forest is described. Optimum response appears at day 5–6 of culture. The cells responsible for lytic activity are nonadherent, Φ-positive, Ig−, and mainly Ly-2.1 positive. Out of five haplotypes tested (H- 2 d ,H- 2 b ,H- 2 s ,H- 2 q , andH- 2 k ) onlyH- 2 k was a responder. Genetic mapping of the response located it solely in theD region of theH- 2 complex. The other four haplotypes responded with a high antiself activity after a second stimulation with viruses. This antiself response also maps in theD region of theH- 2 complex. No complementation was observed in F1 hybrids between responder and nonresponder strains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01844044
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  • 2
    Electronic Resource
    Electronic Resource
    Alloreactive Cytotoxic T Lymphocytes Lyse Syngeneic Influenza-Infected Tumour Cell Targets (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 19 (1984), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Spleen cells from C57BL/10(H-2b) mice, when stimulated in vitro to Kk alloantigens, lysed syngeneic influenza A virus-infected tumour cell targets but not uninfected or vaccinia- or Sendai virus-infected targets. Peritoneal macrophage targets infected with influenza virus were not lysed. Lysis of H-2k targets and EL4-A influenza virus-infected targets was abrogated by treatment of effectors with anti-Thy 1.2plusCandanti-Lyt2plusCbut not by anti-Lyt 1 plusC or anti-GM-1. a natural killer cell-specific, monoclonal antibody plus C. Cold target inhibition experiments indicated that one and the same population of Tc cells see H-2Kk and H-2b plus influenza virus. Sensitization of C57BL/10 mice to Kk in vivo did not potentiate virus clearance from lungs. The data are discussed in relation to observed Ir gene effects and variation and modulation of H-2 antigens on tumour cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1984.tb00943.x
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  • 3
    Electronic Resource
    Electronic Resource
    Effector Cytolotic Funtion but not IFN-γ Production in Cytotoxic T Cells Triggered by Virus-Infected Target Cells In vitro (2001)
    Oxford, UK : Blackwell Science Ltd
    Scandinavian journal of immunology 54 (2001), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Cytolysis and interferon(IFN)-γ production are two independent effector functions of activated cytotoxic T (Tc) cells. We have used the Tc-cell response against the flavivirus, Murray Valley encephalitis virus (MVE), to investigate the requirements for inducing these two functions with regard to antigen-concentration and CD8 coreceptor involvement. Cognate peptide-pulsed target cells triggered cytolysis by primary ex vivo MVE-immune as well as in vitro peptide-restimulated splenocytes at lower peptide concentrations than IFNγ-production (100-fold lower in the case of primary ex vivo effectors). Little difference was observed in CD8 dependency. Importantly, neither of the effector populations were triggered to produce IFN-γ by virus-infected target cells, although cytolysis occurred. This result raises the posibility that the levels of presentation of cognate antigen on virus-infected cells in vivo may be below the threshold required for the IFN-γ production.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3083.2001.00955.x
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  • 4
    Electronic Resource
    Electronic Resource
    Target Cell Lysis by Natural Killer Cells is Influenced by β2-Microglobulin Expression (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 30 (1989), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Natural killer (NK) cells form part of the vertebrate defence against viruses and tumours, but show only limited specificity. The molecule(s) recognized by NK cells on target cells are at present unknown. Major histocompatibility complex (MHC) class I antigen concentration on target cells is inversely correlated with NK cell lysis. Here we show that MHC class I-unassociated β2-microglobulin (β2-m) expression is involved in NK cell-target cell interaction. Two human MHC class I negative cell lines. Daudi and K562, are differentially susceptible to NK cell lysis. Daudi cells are β2-m-negative and resistant to NK lysis, K562 are β2-m-positive and highly susceptible to lysis by NK cells. Interferon (IFN) treatment augments β2-m expression and NK lysis of K562, but not in Daudi cells. NK cell lysis of K562, but not YAC-1 cells, can be inhibited by monoclonal anti-human β2-m antibody. Furthermore, susceptibility of mouse embryo fibroblasts (MEF) to NK lysis can be increased by infection with recombinant vaccinia virus expressing the human β2-m gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1989.tb01184.x
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  • 5
    Electronic Resource
    Electronic Resource
    Vaccinia-Interleukin 2 Recombinant Virus or Exogenous Interleukin 2 Does Not Alter the Magnitude or Immune Response Gene Defects of the Cytotoxic T-Cell Response to Vaccinia Virus in Vivo (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 29 (1989), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We investigated the role of interleukin 2 (IL-2), a T cell-derived lymphokine, in the generation of in vivo cytotoxic T-cell responses to vaccinia virus. We made use of a recombinant vaccinia virus encoding and expressing the murine IL-2 gene and recombinant IL-2 to test the role of IL-2 in the expression of major histocompatibility complex (MHC) class I determined immune response (Ir) gene defects in the response to vaccinia virus. IL-2 expressed either by the vaccinia virus vector or exogenous IL-2 does not alter Ir gene defects nor does IL-2 under such conditions elevate the cytotoxic T-cell response in general.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1989.tb01092.x
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  • 6
    Electronic Resource
    Electronic Resource
    Natural Killer Cells and Cytotoxic T Cells Induce DNA Fragmentation in Both Human and Murine Target Cells in Vitro (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 30 (1989), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: DNA fragmentation induced by cytolytic lymphocytes in human erythromyeloid cell line K562 and murine T lymphoma cell line YAC-1 was investigated by means of agarose gel electrophoresis. Murine natural killer (NK) and cytotoxic T (Tc) cells induced DNA fragmentation in YAC-1 cells, with the fragments being approximately multiples of 180 bp. More significantly, murine NK cells can induce a similar pattern of DNA fragmentation in human K562 cells. Therefore, cytolytic lymphocytes can induce apoptosis or programmed cell death in human target cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1989.tb01185.x
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  • 7
    Electronic Resource
    Electronic Resource
    Transfer of CD8+ T Cells into SCID Mice and Activation of Memory Virus-Specific Cytotoxic T Cells (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 37 (1993), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The requirements for the activation of naive and memory CD8+ cytotoxic T(Tc) cells into effector virus-specific Tc cells after transferring them into SCID mice were investigated. SCID mice reconstituted with splenocytes or purified CDS+ T cells from naive or influenza-immune syngeneic mice and immunized with influenza virus generated effector Tc cells specific for influenza virus-infected target cells in vitro. The kinetics of Ihe response varied between those two populations. The generation of effector Tc cells after transfer of memory CD8+ T cells indicates that there exists no absolute requirement for “help” in the activation of memory virus-immune Tc cells. However, under the conditions described here the in vitro immunogenic peptide NPP derived from influenza nucleoprotein is not sufficient to elicit a response in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1993.tb03329.x
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  • 8
    Electronic Resource
    Electronic Resource
    High Peptide Affinity for MHC Class I does not Correlate with Immunodominance (1999)
    Oxford, U.K. and Cambridge, USA : Blackwell Science Ltd
    Scandinavian journal of immunology 50 (1999), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Cytotoxic T (Tc)-cell responses against influenza virus infection in BALB/c (H-2d) mice are dominated by Tc clones reactive to the viral nucleoprotein (NP). Here, we report investigations using recombinant vaccinia viruses (VV) encoding major histocompatibility complex (MHC) class I H-2Kd molecules differing by a single amino acid from glutamine (wild-type, Kdw) to histidine (mutant, Kdm) at position 114 located in the floor of the peptide-binding groove. Influenza-infected target cells expressing Kdw were strongly lysed by Kd-restricted Tc cells against A/WSN influenza virus or the immunodominant peptide of viral NP (NPP147–155), whereas infected Kdm-expressing targets gave little or no lysis, respectively, thus showing the immunodominance of NPP147–155. Kdm-expressing target cells saturated with synthetic NPP147–155 (10−5 m) were lysed similarly to Kdw-expressing targets by NPP147–155-specific Tc cells. Thus the defect in influenza-infected Kdm-expressing targets was quantitative; insufficient Kdm–peptide complexes were expressed. Tc-cell responses against four other viruses or alloantigens showed no effect of Kdm. When peptide transport-defective cells were infected with VV-Kdw or VV-Kdm and co-infected with a recombinant VV encoding an endoplasmic reticulum-targeted viral peptide, two influenza haemaglutinin peptides caused higher expression of Kdw than NPP147–155 indicating their higher affinity for Kdw. These results are inconsistent with the hypothesis that immunodominance in the anti-influenza response reflects high affinity of the immunodominant peptide, but are consistent with skewing of the Tc-cell receptor repertoire.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3083.1999.00619.x
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  • 9
    Electronic Resource
    Electronic Resource
    West Nile Virus Infection Modulates the Expression of Class I and Class II MHC Antigens on Astrocytes in Vitro (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 540 (1988), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1988.tb27143.x
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  • 10
    Electronic Resource
    Electronic Resource
    Differential target cell susceptibility to SFV-immune cytotoxic T-cells (1989)
    Springer
    Archives of virology 107 (1989), S. 97-109 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Semliki Forest virus (SFV) infects a variety of murine cell types of H-2k haplotype. However, only L 929 fibroblasts can be productively infected. Thioglycollate-induced peritoneal macrophages (TGM), and BW 5147 thymoma cells can be infected with SFV as demonstrated by SFV antigen expression on the cell surface and intracellularly. SFV-immune cytotoxic T (Tc) cells lyse only infected TGM and L929 target cells, however different times after infection are required for lysis of these two cell types. Split clone limiting dilution and cold target competition experiments are consistent with the interpretation that these two cell types display qualitatively different epitopes to SFV-immune Tc cells but do not exclude additional quantitative differences.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01313882
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