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  • 1
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    Linear Precision Glycomacromolecules with Varying Interligand Spacing and Linker Functionalities Binding to Concanavalin A and the Bacterial Lectin FimH (2017)
    Details
    Publication Date: 2020-03-11
    Language: English
    Type: article , doc-type:article
    Library Location Call Number Volume/Issue/Year Availability
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    OPUS
  • 2
    Electronic Resource
    Electronic Resource
    Extraction of capsaicinoids from chillies (Capsicum frutescens L.) and paprika (Capsicum annuum L.) using supercritical fluids and organic solvents (1997)
    Springer
    Zeitschrift für Lebensmittel-Untersuchung und -Forschung 204 (1997), S. 351-355 
    Details
    ISSN: 1431-4630
    Keywords: Key words Supercritical fluid extraction ; HPLC ; Capsaicinoids ; Paprika and chillie powder ; Organic solvent extraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  Supercritical fluid extraction (SFE) with subsequent HPLC analysis was performed in order to determine the amount of capsaicinoids in paprika and chillie powder samples. The extraction yields obtained by SFE were compared to those obtained by organic solvent extraction and were found to be comparable or slightly lower. The advantages of SFE are shorter extraction times, less sample preparation needed prior to HPLC analysis and fewer interfering peaks in the chromatograms. In addition, the SFE method developed (extraction temperature=80 °C, density= 0.75 g/ml, modifier=20 μl water) was shown to be suitable for analysis of capsaicinoids (capsaicin, dihydrocapsaicin, nordihydrocapsaicin) that are present over a wide range of concentrations (10–1400 μg/g) in the samples.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002170050089
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Adaptation of the bacterial community to mercury contamination (2001)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 204 (2001), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The utilisation of 31 sole carbon sources by bacterial communities of soil in the presence of increasing concentrations of Hg(II) was measured by a colour development assay. The assay was performed on Biolog microtitre plates (Ecoplates) in the presence of Hg(II) and compared to Hg(II)-free Ecoplates. Furthermore, community tolerance to Hg(II) was measured by colour development in microtitre plates supplemented with LB broth and by enumeration of colony-forming units on LB agar plates. Both microtitre plates supplemented with LB and LB agar plates contained increasing concentrations of Hg(II). The difference in substrate utilisation profile, as shown by growth on 31 different carbon substrates in the Ecoplates, suggested an adaptation of the soil community that correlated with the metal exposure level in the soil. Similarly, growth on microtitre plates supplemented with LB and plate-spreading data showed an increased community tolerance with increasing levels of mercury in the soil. Both the multi-function microtitre plate assay (Ecoplate) and the LB broth microtitre plate assay are suitable for evaluating the adaptation of the bacterial community in soil to a heavy metal pollutant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2001.tb10861.x
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    The effect of long-term mercury pollution on the soil microbial community (2001)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 36 (2001), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The effect of long-term exposure to mercury on the soil microbial community was investigated in soil from three different sites along a pollution gradient. The amount of total and bioavailable mercury was negatively correlated to the distance from the center of contamination. The size of the bacterial and protozoan populations was reduced in the most contaminated soil, whereas there was no significant difference in fungal biomass measured as chitinase activity. Based on the number of colony morphotypes, moreover, the culturable bacterial population was structurally less diverse and contained a higher proportion of resistant and fast-growing forms. The profiles of amplified 16S rDNA sequences obtained from community DNA by denaturating gradient gel electrophoresis (DGGE) also reflected the altered community structure and decreased diversity along the mercury gradient as expressed in terms of the number and abundance of bands. The functional potential of the microbial population measured as sole carbon source utilization by Ecoplates® differed between the soils, but there was no change in the number of substrates utilized. The observed changes in the different soil microbial populations are probably a combination of both direct and indirect effects of the mercury contamination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.2001.tb00821.x
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    A simple method of sample preparation for analysis of biphenyl residues in citrus fruit peels by gas chromatography (1994)
    Springer
    European food research and technology 198 (1994), S. 329-330 
    Details
    ISSN: 1438-2385
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Description / Table of Contents: Abstract A simple method of sample preparation for analysis of residues of the fungicide biphenyl in peels of citrus fruits by gas chromatography (GC) is described. The blended peels are macerated after addition of anhydrous sodium sulphate in dichloromethane for 2 h. After filtration the biphenyl is quantified directly without concentration of the solution by GC with a flame ionization detector (FID) or a mass selective detector (MSD) under single ion mass conditions. Due to the simple method of sample preparation and good average recoveries of 98–103% with standard deviations of 1–4% in the concentration range of 1–10 ppm, the method described is reliable for analysis of biphenyl in citrus fruit peels. In order to avoid loss of the relatively volatile biphenyl no evaporation of the extraction solution occurs.
    Notes: Zusammenfassung Beschrieben wird eine Probenvorbe-reitungsmethode zur Rückstandsanalyse des Fungizids Biphenyl in Schalen von Citrusfrüchten. Die homogenisierten Schalen werden zwei Stunden lang mit wasserfreiem Natriumsulfat in Dichlormethan maceriert und das Biphenyl ohne vorherige Konzentrierung mit Hilfe der Gaschromatographie (GC) mit einem Flammenionisationsdetektor (FID) oder massenselektiven Detektor (MSD-SIM) quantifiziert. Die Methode zeichnet sich durch hohe Wiederfmdungsraten (98–103%) im Konzentrationsbereich von 1–10 ppm mit Standardabweichungen von 1–4% aus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01193184
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Results of collaborative trials concerning the analysis of benzo-a-pyrene in creosote (1997)
    Springer
    Fresenius' journal of analytical chemistry 357 (1997), S. 1076-1080 
    Details
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract  The results of five collaborative studies concerning the analysis of benzo-a-pyrene (BaP) in creosote by high-performance liquid chromatography (HPLC) are presented. Six to twelve laboratories participated in these studies over a time period of three years. These studies were carried out in order to evaluate the method which is described in the CEN standard ENV 1014-3. The concentrations of BaP in creosote samples analysed were in the range of 20 ppm to 550 ppm. Whereas the values for repeatability (5–10%) were acceptable in nearly all collaborative trials, those for reproducibility have improved significantly in the last study (23–33%). For the previous four collaborative trials the reasons for the relatively poor reproducibility values, especially for analytes containing low concentrations of BaP, could be detected. They were mainly based on problems with chromatographic separation and with integration. The method described in ENV 1014-3 has been proven to be suitable for the determination of the BaP content in creosote.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002160050308
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    Paper (German National Licenses)
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