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  • 1
    Electronic Resource
    Electronic Resource
    Mouse/human chimeric IgGl and IgG4 antibodies directed to the house dust mite allergen Der p 2: use in quantification of allergen specific IgG (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 27 (1997), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background and Objective Chimeric mouse/human monoclonal IgGl and IgG4 antibodies were developed against the house dust mite allergen Der p 2. These chimeric IgG antibodies, hIgG1-Dp2 A and hIgG4-Dp2 A, have the same binding characteristics as the previously reported chimeric hIgE-Dp2 A and are composed of the heavy chain variable domains and light chains ot the original murine monoclonal antibody 2B12., whereas the heavy chain constant domains have been replaced by the human IgGl or IgG4 heavy chain. The expression level of hIgG1-Dp2 A and hIgG4-Dp2 A was 1 and 3.5 μg/mL, respectively.Methods and Results Since all IgG in these culture supernatants is allergen-specific. they are useful reference reagents and enable the calculation of the amount of allergen specific IgG l and IgG4 antibodies in absolute IgG amounts. The results obtained with two panels of sera from patients in immunotherapeutic treatment were evaluated and compared in Der p 2 IgE, IgGl and IgG4 RAST and with reversed lgG4 RAST using labelled purified Der p 2. Close agreement between the results for the two IgG4 assays was found.Conclusion With these chimeric reference reagents the quantities of isotype specific antiallergen antibodies can be calculated and compared.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.1997.tb01262.x
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  • 2
    Electronic Resource
    Electronic Resource
    Phänotypische Heterogenität kardialer Myxome (1987)
    Springer
    Journal of molecular medicine 65 (1987), S. 912-919 
    Details
    ISSN: 1432-1440
    Keywords: Heterogeneity of cardiac myxomas ; Immunohistology of cardiac myxomas ; Endothelial characteristics of cardiac myxomas ; Monoclonal antibodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Histogenesis of cardiac myxoma is still unclear. Beside endothelial cells a variety of different cell types were detected in this benign cardiac tumor. Cryostat sections of four myxomas were analysed in the indirect immunoperoxidase technique using monoclonal antibodies (MoABs) directed against MHC class I and II antigens, as well as different surface determinants specific for endothelial cells or monocytes/macrophages. Tumor cells forming cell clusters and blood vessel like structures differed in their expression of endothelial antigens suggesting cellular heterogeneity within single and different myxomas. Like in fetal cardiac tissue vascular channels rarely carried HLA-class II antigens. Tumor cells carrying antigens of monocytes/macrophages, as well as intracellular alkaline phosphatase of endothelial cells could represent subpopulations of an early differentiation stage. This analysis further supports previous hypothesis of an endothelial origin of myxomas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01745502
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  • 3
    Electronic Resource
    Electronic Resource
    Immunogenetics of glomerulonephritis (1993)
    Springer
    Journal of molecular medicine 71 (1993), S. 822-824 
    Details
    ISSN: 1432-1440
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00190329
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  • 4
    Electronic Resource
    Electronic Resource
    The progression of renal diseases: On the pathogenesis of renal interstitial fibrosis (1991)
    Springer
    Journal of molecular medicine 69 (1991), S. 576-586 
    Details
    ISSN: 1432-1440
    Keywords: Renal interstitial fibrosis ; Fibroblast cell system ; Collagen synthesis ; Local immune responses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Renal interstitial fibrosis (RIF) frequently occurs in inflammatory and non-inflammatory kidney diseases and is associated with a decline in renal excretory function. Fibroblasts which occupy the renal interstitium are involved mainly in the formation of RIF not only by the production of extracellular matrix, but also by regulatory processes. They respond to a variety of cytokines released by different cell types. To investigate mechanisms leading to RIF, immunohistochemical analysis and cell cultures of renal biopsies in various renal diseases have been performed. T lymphocytes are the major cells infiltrating the renal interstitium, and their number correlates with the impairment of renal function. In most forms of glomerulonephritis accompanied by interstitial inflammation, an abnormal expression of HLA-DQ/-DP molecules, frequently associated with an aberrant expression of the intercellular adhesion molecule 1 (ICAM-1), was observed on proximal tubular epithelial cells, indicating that these cells may play a role in antigen presentation. The cell biological experiments revealed the presence of the three mitotic fibroblast types (MFI-MFIII) and the three postmitotic types (PMFIV-PMFVI) in the cell culture. The number of fibroblasts in primary and passage-1 culture was increased sevenfold in cultures derived from kidneys with RIF (FKIF cells) in comparison to normal kidneys (NKF cells). FKIF cells show hyperproliferative growth and synthesize an increased amount of total collagen, especially types III and V. These cells express a protein, named “FIBROSIN”, which seems to be specific for FKIF cells. Further extended cell biological analyses are currently being performed to investigate interactions of tubular cells, lymphocytes, macrophages, and fibroblasts in order to shed more light on the pathomechanisms involved in fibrogenesis leading to renal interstitial fibrosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01649320
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  • 5
    Electronic Resource
    Electronic Resource
    Treatment of non anti-GBM-antibody mediated, rapidly progressive glomerulonephritis by plasmapheresis and immunosuppression (1986)
    Springer
    Journal of molecular medicine 64 (1986), S. 231-238 
    Details
    ISSN: 1432-1440
    Keywords: Rapidly progressive glomerulonephritis ; Prognosis ; Immunosuppressive drugs ; Plasma exchange ; Interstitial fibrosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A retrospective study was conducted to evaluate the efficacy of plasmapheresis in combination with different immunosuppressive drugs (“pulse” therapy, azathioprine or cyclophosphamide together with steroids) in nine patients presenting with rapidly progressive glomerulonephritis (RPGN) not mediated by antibody to glomerular basement membrane. Six of these patients had to be initially dialysed. All patients underwent renal biopsy, which revealed that seven patients had a minimum of 80% crescents and five had interstitial fibrosis. Recovery of renal function was observed in seven patients (78%). All patients without interstitial fibrosis were recompensated for at least 14 months after the acute onset of RPGN. Those who presented with interstitial fibrosis declined to endstage renal failure after 13 months requiring chronic hemodialysis treatment or cadaveric kidney transplantation. On the basis of these findings interstitial fibrosis seems to be a limiting factor for the prognosis of non-anti-GBM-antibody mediated RPGN.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01711654
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  • 6
    Electronic Resource
    Electronic Resource
    Relationship of antibodies to endotoxin core to mortality in medical patients with sepsis syndrome (1999)
    Springer
    Intensive care medicine 25 (1999), S. 435-444 
    Details
    ISSN: 1432-1238
    Keywords: Key words Sepsis syndrome ; Medical intensive care ; Endotoxin ; Endotoxin core antibodies ; Sepsis score ; Prognostic factors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Objectives: To assess the prognostic value of determining anti-endotoxin core antibodies (EndoCab) immunoglobulin (Ig)G and IgM in medical patients with sepsis syndrome in order to identify patient subgroups that may profit from endotoxin-neutralizing therapy. The findings were correlated with clinical outcome, endotoxin levels and sepsis score. Design: Cohort study with a follow-up period of 30 days. Setting: Medical intensive care units (2) of a university hospital. Patients and methods: Twenty-nine patients who fulfilled the criteria of sepsis syndrome and did not present with septic shock or had not been treated with antibiotics for more than 3 days were included in the study. Twenty-one intensive care patients without infections served as controls for antibody concentrations. Interventions: Blood samples were obtained from indwelling arterial catheters or direct venipuncture on admission and daily thereafter until transfer to a regular unit. Sepsis scores were determined daily. Results: The mortality rate at 30 days was 44.8 % (13 out of 29). Sepsis patients had significantly lower initial EndoCab IgM and IgG concentrations than controls. Initial EndoCab IgG concentrations were significantly lower in non-survivors of sepsis syndrome but not in survivors compared to controls (median concentrations 51.5 vs 110.1 vs 245.4 MU/ml). EndoCab IgM and IgG were lower in non-survivors compared to survivors, though that difference failed to reach significance (p = 0.11 in both cases). Depletion of initial EndoCab IgM concentrations (defined as a value below the 10th percentile of a control population) was present in 15 patients, 9 of whom died, and depletion of IgG in five patients, four of whom died. EndoCab IgM and IgG concentrations rose concordantly in survivors and non-survivors in the course of the disease. Endotoxin levels were significantly higher in non-survivors compared to controls but not in survivors. A sepsis score of 21 and higher was associated with 90.9 % mortality (specificity 93.8 %, sensitivity 76.9 %). Conclusions: Decreased EndoCab IgG concentrations are associated with increased mortality in medical patients with sepsis syndrome. The measurement of initial anti-endotoxin antibodies may provide a useful tool to identify septic patients who profit potentially from endotoxin neutralizing therapy, however considerable overlap of antibody concentrations warrants additional parameters. The sepsis score is easy to determine and useful in the evaluation of medical patients with sepsis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001340050877
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  • 7
    Electronic Resource
    Electronic Resource
    17jährige Patientin mit Ganzkörpererythem, Fieber, kompensierte Niereninsuffizienz, Thrombozytopenie und erhöhter Kreatinkinase (1997)
    Springer
    Der Internist 38 (1997), S. 251-254 
    Details
    ISSN: 1432-1289
    Keywords: Schlüsselwörter Toxisches Schock-Syndrom (TSS) ; Diagnosekriterien des TSS ; Superantigene ; Toxine produzierende Staph. aureus- und Streptokokkenstämme ; TSS-ähnliches Syndrom
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Zusammenfassung Eine 17jährige Patientin mit Ganzkörpererythem, Fieber, kompensierter Niereninsuffizienz, Thrombozytopenie und erhöhter Kreatinkinase wurde stationär aufgenommen. Es ließ sich die Diagnose eines toxischen Schock-Syndromes stellen, ausgelöst durch von Staphylkokkus aureus produzierten Toxinen. Die Patientin bot eine klassische Klinik sowie die entsprechende Laborkonstellation. Die Pathogenese, Therapie und Differentialdiagnose des bereits 1978 von James Todd erstmals beschriebenen toxischen Schocksyndromes werden diskutiert.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001080050038
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  • 8
    Electronic Resource
    Electronic Resource
    Diuretika bei Herzinsuffizienz (1998)
    Springer
    Der Internist 39 (1998), S. 320-327 
    Details
    ISSN: 1432-1289
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001080050174
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  • 9
    Electronic Resource
    Electronic Resource
    Demonstration of a Na+-dicarboxylate cotransporter in bovine adrenocortical cells (1999)
    Springer
    Pflügers Archiv 438 (1999), S. 860-864 
    Details
    ISSN: 1432-2013
    Keywords: Dicarboxylate transporter Succinate uptake Citrate pH-Dependency 2,3-Dimethylsuccinate Bovine adrenocortical cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Our study found the uptake of [14C]succinate into bovine adrenocortical cells to be sodium-dependent, inhibited by lithium, and to have an apparent K m of 146 µmol/l. Succinate uptake was inhibited by glutarate, fumarate, α-ketoglutarate, and maleate but not by 2,3-dimethylsuccinate or cis-aconitate, specific inhibitors of the basolateral Na+-dicarboxylate transporter of renal proximal tubule cells. Succinate uptake was highest at pH 6.0 and decreased with increasing pH. Transport of succinate was not significantly inhibited by citrate at pH 7.4 whereas at pH 6.0 inhibition of succinate uptake by citrate was small but significant. The affinity of the adrenal dicarboxylate transporter towards succinate ranges in between the low affinity of the renal luminal dicarboxylate transporter and the high affinity of the respective basolateral transporter. The pH dependency of succinate uptake and the missing inhibition by citrate at pH 7.4 differ from both the luminal and from the basolateral dicarboxylate transporters in kidney, liver, intestine, and placenta. These functional characteristics provide evidence for the existence of a Na+-dicarboxylate cotransporter in adrenocortical cells which may supply cholesterol metabolism with reducing substrates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004249900148
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  • 10
    Electronic Resource
    Electronic Resource
    Pharmacokinetics of torasemide and its metabolites in end-stage renal disease (1994)
    Springer
    European journal of clinical pharmacology 47 (1994), S. 157-159 
    Details
    ISSN: 1432-1041
    Keywords: Torasemide ; metabolites ; end-stage renal disease ; pharmacokinetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Abstract The pharmacokinetics of torasemide, a new loop diuretic, as well as its active metabolites M1 and M3, and its inactive main metabolite, M5, were studied in 12 patients with end-stage renal failure during single i.v. (n=6) or single oral (n=6) dosing of 200 mg torasemide, and during chronic oral treatment for 9 days (n=12). The elimination half-life (t1/2) of torasemide was unchanged in renal failure, whereas t1/2 of the torasemide metabolites M1, M3, and M5 were markedly prolonged. However t1/2 as well as the area under the plasma level time curve of torasemide and its metabolites were unchanged during chronic compared to acute administration. The results of this study suggest that despite the increased half-life of torasemide metabolites M1, M3 and M5 in end-stage renal failure patients, no accumulation of the parent drug torasemide and its metabolites during chronic dosing is demonstrable.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00194966
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