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  • 1
    Electronic Resource
    Electronic Resource
    Influence of maternal immunity on the outcome of mutine coronavirus JHM infection in suckling mice (1985)
    Springer
    Medical microbiology and immunology 174 (1985), S. 15-24 
    Details
    ISSN: 1432-1831
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Adult C3H mice are resistant to intraperitoneal infection with murine coronavirus JHM, whereas suckling offspring of non-immune females are susceptible. Resistance can be conferred on suckling C3H mice by postnatal transmission of maternal immunity, if transfer preceeds infection. Suckling mice succumb to infection even when they receive maternal antibodies within 1 day after infection. Prenatal transmission alone without subsequent postnatal transmission of maternal immunity is not sufficient to provide resistance. Persistence of virus without clinical consequences was observed when the supply of breast milk anti-JHMV antibodies was terminated 5 days before infection. Immune reactions restricted by histocompatibility antigens do not play a crucial role in bestowing resistance. As neutralizing anti-JHM serum antibody titers of adult mice only rise sharply 5 to 7 days after infection, these results indicate that infection of adults can be arrested by immunological means but that, in addition, the rate of virus dissemination must be limited by other non-immunological mechanisms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02123666
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The catecholaminergic system of an annelid (Ophryotrocha puerilis, Polychaeta) (1991)
    Springer
    Cell & tissue research 265 (1991), S. 175-184 
    Details
    ISSN: 1432-0878
    Keywords: Nervous system ; Nervous system, peripheral ; Catecholamines ; Immunohistochemistry ; Glyoxylic acid fluorescence ; Ophryotrocha puerilis (Annelida)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The complex catecholaminergic (CA) nervous system of the polychaete Ophryotrocha puerilis is documented using glyoxylic acid induced fluorescence (GIF) and immunohistochemistry. CA-neurons are found both in the central and peripheral nervous system. In the brain, about 50 CA-neurons are present in the perikaryal layer together with numerous CA fibres. Two pairs of CA perikarya are characteristic for each ganglion of the ventral nerve cord. CA-neurites in the ventral nerve cord are mainly arranged in 4 strands paralleling the longitudinal axis of the worm. Fluorescent neurons with receptive ciliary structures are present in body appendages (antennae, palps, urites, parapodial cirri), in the body-wall, and within the oesophageal wall. Furthermore, a subepidermal nerve net of free CA nerve endings has been found. After incubation of specimens with dopamine prior to the development of GIF more fluorescent perikarya could be observed; the fluorescence was also intensified. Pre-incubation with reserpine reduced the intensity of GIF. Results of high pressure liquid chromatography and immunostaining with a polyclonal antibody against a dopamine-glutaraldehyde-complex suggest that dopamine is the major CA transmitter. It is thought that dopaminergic neurons together with ciliary receptive structures act as mechano- and/or chemoreceptors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318152
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Cytophysiology of neurosecretory axon terminals in the brain of an annelid (Ophryotrocha puerilis, Polychaeta) (1991)
    Springer
    Cell & tissue research 264 (1991), S. 339-345 
    Details
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Catecholamines ; HPLC ; Immunohistochemistry ; Glyoxylic acid fluorescence ; Ophryotrocha puerilis (Annelida)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the posterior part of the brain of the protandric polychaete Ophryotrocha puerilis neurosecretory cells form prominent axon terminals. The terminals are arranged in two complexes. The perikarya of these presumably monopolar neurons are scattered in the anterior part of the cerebral perikaryal layer. In females the terminals store large amounts of neurosecretory material. It has been suggested earlier that neurosecretions of the terminals may play a role during sex reversal from females to males. Application of histamine caused the release of neurosecretory material from the respective terminals in females. However, this discharge was not followed by sex reversal. Application of reserpine had no influence on the terminals. Neither by in vivo observation nor by ultrastructural analysis any effect of reserpine on the terminal complexes could be observed. In isolated terminals filled with neurosecretory material from females, catecholamines could not be detected by HPLC. Also, polyclonal antibodies against dopamine did not stain the terminal complexes. Furthermore, the complexes did not develop any fluorescence after glyoxylic acid treatment. Therefore, the present results contradict the hypothesis that the neurosecretory material of the respective axon terminals is catecholaminergic and that it is involved in sex differentiation. The function of the secretory neurons studied here remains unclear.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00313972
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Das Agglutinin-Anreicherungsverfahren, ein neues Verfahren zur Blutgruppenbestimmung an altem, eingetrocknetem Blute (1928)
    Springer
    International journal of legal medicine 11 (1928), S. 120-128 
    Details
    ISSN: 1437-1596
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Zusammenfassung 1. Beschreibung eines neuen Verfahrens zur Blutgruppenbestimmung bei altem, eingetrocknetem Blute. Wir bezeichnen die neue Methode alsAgglutinin-Anreicherungsverfahren. 2. Bei den ausgeführten 80 Versuchen wurde in ca. 70% der Fälle ein positives Resultat erzielt. Das Alter der Blutproben, das sich zwischen 1/2–18 Monaten bewegte, hatte keinen sichtbaren Einfluß auf die Intensität der Agglutination. 3. Gestützt auf die guten Resultate, wird die Blutgruppenuntersuchung bei eingetrocknetem Blute zu gerichtlich-medizinischen Zwecken empfohlen. 4. Es wird gleichzeitig mit demAgglutinin-Anreicherungsverfahren die Blutgruppenbestimmung bei allen Verurteilten empfohlen, als wertvolle Bereicherung des Signalements von Verbrechern.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01748803
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Standard addition for strongly non-linear calibration curves (1981)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 307 (1981), S. 109-114 
    Details
    ISSN: 1618-2650
    Keywords: Eichzusatzmethode ; stark gekrümmte Eichkurven ; Densitometrie ; FORTRAN-Programm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary An iterative procedure has been applied to standard addition in order to overcome limitations so far involved with the standard addition technique. Using a FORTRAN-program even strongly nonlinear calibration functions could be evaluated. Moreover, in contrast to linear standard addition techniques remaining background absorption does hardly influence the result. Up to now this program has just been applied to densitometric data, but scientists are encouraged to use it whenever a standard addition technique is to be applied and calibration curves are not linear or background noise is observed.
    Notes: Zusammenfassung Der Einsatzbereich der Eichzusatzmethode konnte durch Anwendung eines iterativen Rechenverfahrens bedeutend vergrößert werden. Mit Hilfe eines FORTRAN-Programms wurde es möglich, stark gekrümmte Eichfunktionen der Eichzusatzmethode zugänglich zu machen. Darüber hinaus zeigt sich, daß eine Verschiebung der Eichkurve durch unspezifische Untergrundabsorption kaum Einfluß auf das analytische Ergebnis hat. Bislang wurde das Programm nur zur Auswertung densitometrisch gewonnener Daten benutzt. Dennoch möchten wir es auch für andere Analysenverfahren empfehlen, wenn eine Eichzusatzmethode bei nicht linearen Eichkurven eingesetzt werden soll. Bei Grundrauschen kann das Programm ebenfalls vorteilhaft eingesetzt werden.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00487489
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    Paper (German National Licenses)
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