ISSN:
1420-908X
Keywords:
Rheumatoid arthritis
;
Methotrexate
;
Gold salts
;
Interleukin-10
;
Interleukin-6
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract In order to evaluate the relationship between serum concentrations of interleukin-10 (IL-10), IL-6, and acute phase proteins in rheumatoid arthritis (RA) patients treated with methotrexate (MTX) or intramuscular gold (IMG) we determined IL-10, IL-6, C-reactive protein (CRP), alpha-1-acid glycoprotein (AGP) and alpha-1-antichymotrypsin (ACT) in the sera of 35 RA patients. IL-10 and IL-6 levels were evaluated using an enzyme-linked immunoassay (ELISA). AGP and ACT level were measured using rocket immunoelectrophoresis. IL-10 serum level was not increased in RA patients as compared to controls (58.7 ± 18.1 pg/ml vs. 57.2 ± 11.9 pg/ml). IL-6 level was significantly elevated (91.6 ± 46.9 pg/ml vs. 45 ± 19 pg/ml, p 〈 0.05). CRP was significantly increased as compared to healthy controls (35 ± 19 mg/l vs. 3 ± 2 mg/l, p 〈 0.05). Patients treated with MTX or IMG presented an increased level of IL-10 and decreased amounts of IL-6, as compared to those treated with NSAID only. However, only changes between patients treated with IMG and NSAID were found to be statistically significant. A good negative correlation between IL-10 and IL-6 serum level was found (r = −0.75, p 〈 0.05). A positive significant correlation between IL-6 serum level and CRP (r = 0.62, p 〈 0.05), AGP (r = 0.78, p 〈 0.05) and ACT (r = 0.45, p 〈 0.05) was established. On the other hand, a negative correlation between IL-10 and serum level of CRP (r = −0.76, p 〈 0.05), AGP (r = −0.64, p 〈 0.05) and ACT (r = −0.38, p 〈 0.05) was also observed. Moreover, these relationships were maintained when patients treated with MTX, IMG, or NSAID were analyzed independently. According to the data thus far obtained, it seems that IL-10 decreases IL-6 production, and thereby indirectly affects the acute phase response, decreasing CRP, AGP, and ACT concentration in RA patients.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01630483
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