ISSN:
1745-4549
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
A new process for preparing enriched fractions of α-lactalbumin (α-La) and β-lactoglobulin (β-Lg) from whey protein concentrate was developed. To prepare the fractions, solutions containing from 7% (w/w) to 25% (w/w) whey protein concentrate (WPC75) were sparged with carbon dioxide (CO2) in a batch reactor. The effects of pressure, temperature, concentration, and residence time on the distribution of the individual whey proteins in each fraction were investigated. Best results were obtained for 7% (w/w) WPC75 solutions, at reactor fill pressures of 4140 kPa and 5520 kPa, temperature of 64C and reactor residence time of 10 min. Gel electrophoresis of unpurified enriched samples showed that recovery of α-La was approximately 55% and that of β-Lg was 78%. The resulting fractions have a pH of 6.0 and contain no added salts.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1745-4549.1998.tb00363.x
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