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  • 1
    Electronic Resource
    Electronic Resource
    Neurotransmitter Systems in Morphologically Undifferentiated Human Y-79 Retinoblastoma Cells: Studies of GABAergic, Glycinergic, and β-Adrenergic Systems (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 45 (1985), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Elements of three neurotransmitter systems were investigated in morphologically undifferentiated human Y-79 retinoblastoma cells in suspension culture. Specific γ-aminobutyric acid (GABA) uptake, GABA binding, and glycine binding were absent from these cells, although the cells had been shown to exhibit an active uptake and release of [3H]glycine. Binding and competition studies using both α-and β-adrenergic ligands indicated the presence of a β-adrenergic receptor. This finding was confirmed by treatment of the cells with β-agonists in competition with a β-antagonist and with an α-antagonist; the level of cyclic AMP was competitively stimulated. Therefore, human Y-79 cells in suspension culture contain β-adrenergic receptors, and not glycinergic or GABAergic ones. Thus, the Y-79 cells may be of use in studying the factors involved in developmental regulation of neurotransmitter function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1985.tb10541.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Chloride Ions Preferentially Mask High-Affinity GABA Binding Sites (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 43 (1984), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Chloride ions (Cl−) act to regulate the appearance of γ-aminobutyric acid (GABA) binding sites in synaptic membranes from bovine retinas. Scatchard analysis indicates that the presence of Cl−results in a preferential decrease (85%) in the number of high-affinity binding sites, with no statistically significant change in the affinity (KD) of either the high-or low-affinity sites. The finding that the appearance of this binding site is altered by the presence of Cl−may indicate that Cl−acts, either directly or indirectly, to regulate the appearance, and hence function, of the high-affinity GABA receptor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1984.tb05405.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    GABA receptor binding site “induction” in rabbit retina after nipecotic acid treatment: Changes during postnatal development (1986)
    Springer
    Neurochemical research 11 (1986), S. 55-61 
    Details
    ISSN: 1573-6903
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The development of the GABA system in the rabbit retina was studied. The number of high- and low-affinity GABA receptor binding sites increased in a sigmoidal manner, with the curve for the low-affinity sites lagging 2–3 days behind that for the high-affinity sites. TheK D for both high-(17.5 nM) and low-affinity (138.0 nM) sites remained constant during development. Treatment of isolated eyecups with the uptake blocker nipecotic acid resulted in an increase in theB max for high-affinity sites in developing tissue with the maximum sensitivity around eye opening; mature tissue exhibited a decrease inB max. In contrast, a gradual decrease in sensitivity to stimulation of the low-affinity sites occurred. These data indicate that the “trophic” action of GABA is limited to the time when the tissue is developing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00965165
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    [3H]GABA binding in developing rabbit retina (1982)
    Springer
    Neurochemical research 7 (1982), S. 495-503 
    Details
    ISSN: 1573-6903
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We have studied the developmental sequence of the GABA system in the rabbit retina using an in vitro binding assay to monitor developmental changes in the post-synaptic receptor. A variety of tissue treatments including perchlorate and Triton X-100 were employed to optimize binding and remove endogenous factors which inhibit binding. Pre-treatment of the tissue with 0.05% Triton X-100 revealed high affinity binding for [3H]GABA which increased in a sigmoidal fashion with the post-natal age of the animal. A constant level of binding, at about 16% of adult levels, was noted until day 8, at which time a rapid increase occurred. At 16 days post-natal, the amount of specific binding reached a plateau near adult levels. Kinetic analysis of the GABA receptor showed an increase in the number of receptors (Bmax) with little or no change in the apparent affinity (KD). Our results suggest that the onset of post-synaptic receptor activity is delayed approximately 1 to 2 days, relative to the pre-synaptic components, and the period of rapid increase in GABA receptor binding coincides with the period of maximum increase in retinal synaptic density.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00965501
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    Paper (German National Licenses)
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