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  • 1
    Publication Date: 2017-11-15
    Description: Pattern Datenbanken (PDBs) werden in der heuristischen Suche eingesetzt, um irrelevante Pfade in der Suche auszuschließen. Da die Leistung einer PDB mit ihrer Größe steigt und für die effektive heuristische Suche in großen Zustandsräumen große PDBs nötig sind, ist ein paralleler Ansatz notwendig, um sehr große PDBs zu erzeugen. Diese Arbeit erweitert einen, auf MapReduce basierenden, Algorithmus von Reinefeld und Schütt (2009) zur massiv-parallelen Breitensuche so, dass damit sehr große PDBs erzeugt werden können. So entsteht die erste vollständige 8+8+8 PDB für das 24er-Puzzle. Reinefeld und Schütt implementieren ebenfalls das heuristische Suchverfahren BFIDA* für MapReduce. In dieser Arbeit wird dieser Implementation ein Speedup von 857 bei 2039 Kernen nachgewiesen. Weiterhin führt diese Arbeit ein Schema ein, mit dem PDBs bei der Suche direkt von der Festplatte gelesen werden können. Des Weiteren wird der Nutzen von großen PDBs in einer Gruppe von PDBs untersucht, deren Heuristikwerte maximiert werden. Dazu wird analysiert, wie sich Gruppen von PDBs mit unterschiedlicher Größe verhalten und welche Faktoren bei solchen Konstellationen zum Erfolg der Gruppe beitragen. Es wird gezeigt, dass der Einsatz von großen PDBs effizient ist, wenn der zur Verfügung stehende Hauptspeicher ausreicht, eine Gruppe zusammen mit einigen kleineren PDBs zu bilden.
    Language: German
    Type: masterthesis , doc-type:masterThesis
    Format: application/pdf
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  • 2
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of agricultural and food chemistry 15 (1967), S. 113-117 
    ISSN: 1520-5118
    Source: ACS Legacy Archives
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-072X
    Keywords: Hydrogenase ; Ribulosebisphosphate carboxylase ; Hydrogen uptake ; Rhizobium japonicum, CO2 fixation ; Propionyl coenzyme A carboxylase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract H2-uptake positive strains (122 DES and SR) and H2-uptake negative strains SR2 and SR3 of Rhizobium japonicum were examined for ribulosebisphosphate (RuBP) carboxylase and H2-uptake activities during growth conditions which induced formation of the hydrogenase system. The rate of 14CO2 uptake by hydrogenase-derepressed cells was about 6-times greater in the presence than in the absence of H2. RuBP carboxylase activity was observed in free-living R. japonicum strains 122 DES or SR only when the cells were derepressed for their hydrogenase system. Hydrogenase and RuBP carboxylase activities were coordinately induced by H2 and both were repressed by added succinate. Hydrogenase-negative mutant strains SR2 and SR3 derived from R. japonicum SR showed no detecyable RuBP carboxylase activities under hydrogenase derepression conditions. No detectable RuBP carboxylase was observed in bacteroids formed by H2-uptake positive strains R. japonicum 122 DES or SR. Propionyl CoA carboxylase activity was consistently observed in extracts of cells from free-living cultures of R. japonicum but activity was not appreciably influenced by the addition of H2. Neither phosphoenolpyruvate carboxylase nor phosphoenolpyruvate carboxykinase activity was detected in extracts of R. japonicum.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 132 (1982), S. 219-224 
    ISSN: 1432-072X
    Keywords: Rhizobium ; Nitrogen fixation ; Nodules ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Several mutants defective in nodulation were isolated from Rhizobium japonicum strains 3I1b110 and 61A 76. Mutants of class I do not form nodules after incubation with soybean [Glycine max (L.) Merrill] for 17 days, but will do so by 28 days. When host plants other than G. max are infected with several of these strains, there is no detectable difference in the time of nodulation or size of nodules as compared to the wild type. Two mutants of class I (i. e., SM1 and SM2) have been shown previously to be altered in the lipopolysaccharide portion of their cell wall. Mutants of class II are not slow to nodulate but form fewer nodules than the wild type on all the host plants tested. Mutants of class III are unable to form nodules. Some bacteriophage-resistant mutants, altered in cell surface structure, fall into this class. Two mutants of class III do not bind to soybean roots.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-072X
    Keywords: Bradyrhizobium japonicum ; Hupc mutants ; Hydrogenase apoprotein ; Nickel metabolism ; Nickel incorporation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A double mutant (JH103K10) was created from hydrogenase constitutive mutant (JH103) by replacement of a chromosomal 0.60 kb nickel metabolism related locus with a kanamycin resistance gene. The double mutant required 10 to 20 times more nickel (Ni) to achieve near parental strain levels of hydrogenase activity. In the absence of nickel, both JH103K10 and JH103 synthesized high levels of (inactive) hydrogenase apoprotein (large subunit, 65 kDa). With nickel, the double mutant JH103K10 synthesized the same level of hydrogenase apoenzyme (65-kDa subunit) as the JH103 parent strain; however, whole cell hydrogenase activity in JH103K10 was less than half of that in JH103, and the CPM (due to 63Ni in hydrogenase) of membranes and the calculated ratio of nickel per unit of hydrogenase enzyme of the double mutant were 40% of that in JH103. Therefore, the difference in hydrogenase activities between the double mutant and the Hupck strain can be accounted for by different abilities of the strains to incorporate nickel into the hydrogenase apoenzyme. The addition of nickel ions to previously Ni-starved and then chloramphenicol-treated Bradyrhizobium japonicum whole cells (JH103 and JH103K10) resulted in (an in vivo) restoration of hydrogenase activity, suggesting that the apoprotein synthesized in the Ni-free cultures could be activated by addition of nickel even in the absence of protein synthesis. The extent of reconstitution of active hydrogenase by nickel was greater in the absence of chloramphenicol. Hydrogenase apoprotein could not be activated by nickel in vitro even with the addition of ATP. The successful in vivo but not in vitro results suggest that enzymatic but cell-disruption labile factors are required for Ni incorporation into hydrogenase.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 160 (1993), S. 43-50 
    ISSN: 1432-072X
    Keywords: Hydrogenase ; Gene expression ; Bradyrhizobium japonicum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plasmid-borne hup-lacZ transcriptional fusion constructs were introduced into three separate mutant strains of Bradyrhizobium japonicum which express hydrogenase constitutively (Hupc strains SR470, SR473 and JH101) in both autotrophic and heterotrophic environments. The lacZ structural gene linked directly to the regulatory region upstream of the hydrogenase structural gene encompassing -149 bases expressed β-gal at a constant, high level, in response to various concentrations of Ni (0 μM to 1 μM). β-Gal activity was expressed at a constant level in response to variations in concentration of O2 (0%–10%) and H2 (0%–10%) as well. The cis-acting region required to express hydrogenase constitutively is located between -149 and -98 bases. This is also the site of nickel, oxygen and hydrogen-dependent regulatory action in the wild-type strain. It is postulated that a single mutation in Hupc strains affects the trans-acting factor which would normally by responsive to Ni, O2 and H2.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 110 (1993), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The Bradyrhizobium japonicum heterodimeric nickel-iron hydrogenase efficiently catalyzed H2-ubiquinone-1 oxidoreductase activity at rates up to 47% of the maximal rates obtained using the artificial electron acceptor methylene blue. Gel filtration chromatography and SDS-polyacrylamide gel electrophoresis experiments demonstrated that the purified enzyme was a heterodimer containing only the 65 kDa and 33 kDa subunits. Reduced minus oxidized absorption difference spectra demonstrated the absence of detectable cytochromes. The H2-ubiquinone-1 oxidoreductase activity of both the purified heterodimeric hydrogenase and membranes was significantly inhibited by 2-n-heptyl-4-hydroxyquinoline-N-oxide and antimycin A, inhibitors known to act in the quinone region of electron transport chains. Our results are the first report of H2-ubiquinone oxidoreductase activity by a purified hydrogenase.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 109 (1993), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Due to the high incidence of spontaneous antibiotic resistance and slow growth of Bradyrhizobium japonicum strains, screening for site-directed mutants is cumbersome and time-consuming. A rapid method for selection of recombinant site-directed mutants of B. japonicum was developed. A kanamycin (Km) and a spectinomycin (Sp) cassette were each used to replace DNA fragments in the chromosome by homologous recombination. The primary new features of this method involve a simple plate selection for the antibiotic (Km or Sp) resistant mutants, then colony streaking, and lysis for DNA hybridization on a nitrocellulose filter enabling direct identification of the recombinant site-directed mutants. This method has permitted us to quickly and easily identify a large number of positive recombinant mutants from a large number of indicidual colonies. The procedure eliminates the need to first isolate genomic DNA from each mutant for Southern hybridization. All of the tested site-directed mutants from this method were confirmed to exhibit the expected mutant phenotype.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 183 (1959), S. 461-462 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] There are several direct photometric methods for the determination of magnesium, of which titan yellow2 and eriochrome black Tz are examples. There is need for a direct titrimetric procedure for magnesium. Chelometric titrations employing ethyl-enediamine tetraacetate are widespread. These methods, ...
    Type of Medium: Electronic Resource
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