ISSN:
1572-9931
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract We have developed an efficient system for obtaining myeloma mutants defective intrans-acting factors required for immunoglobulin (Ig) gene expression. The system consists of a myeloma cell line designed for this purpose and an efficient method for selecting mutants from it. The cell line is X63.653 transfected with the μ gene, whose tailpiece sequence was replaced with the transmembrane sequence of human EGF receptor to hold μ on the cell surface and whose CH1 sequence was removed to prevent μ from being retained in the endoplasmic reticulum. It efficiently and stably expressed μ chains of IgM on the cell surface (μ m + ) without light chains. To obtain mutants lacking μm (μ m − ) from the μ m + cell line by selectively killing μ m + cells, a method with ricin A-conjugated anti-μ antibody was more reliable than complement lysis mediated by anti-μ antibody. Applying the system, we obtained a variety of μ m − mutants.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01232651
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