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  • 1
    Electronic Resource
    Electronic Resource
    Inhibition of spontaneous and experimental tumor metastasis by the calcium antagonist verapamil (1985)
    Springer
    Cancer chemotherapy and pharmacology 14 (1985), S. 30-33 
    Details
    ISSN: 1432-0843
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Verapamil, a calcium antagonist, inhibited both experimental (IV inoculation of tumor cells) and spontaneous metastasis (SC inoculation) of the highly metastatic B16 melanoma and colon adenocarcinoma 26 cell lines. Verapamil treatment resulted in a maximum 80% inhibition of metastases, the degree of inhibition varying among the different metastatic systems. Verapamil inhibited platelet aggregation induced by these tumor cell lines, the patterns of inhibition being different for B16 melanoma and colon adenocarcinoma. The inhibition of platelet aggregation induced by tumor cells is proposed as a mechanism by which the calcium antagonist exerts its antime-tastatic effect. These results, together with our previous findings that calcium antagonists can increase the cytotoxicity of drugs in tumor cells with induced or inherent drug resistance by inhibiting outward transport of the drug, indicate that calcium antagonists have potential as a new class of adjuvant agents in the field of cancer chemotherapy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00552721
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Enhancing effect of mouse peritoneal exudate cells and their products on antibody productivity of hybridoma cells: Application of in vivo factors to in vitro culture (1991)
    Springer
    Cytotechnology 7 (1991), S. 93-101 
    Details
    ISSN: 1573-0778
    Keywords: hybridoma culture ; interleukins ; monoclonal antibody productivity ; peritoneal exudate cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Mouse peritoneal exudate cells induced by casein enhanced in vitro antibody production rate per cell of a hybridoma in co-culture. Culture supernatant of the exudate cells also enhanced three-fold the antibody productivity when added to cultures of a hybridoma at 10% (v/v). Hence the enhancement of antibody productivity by the exudate cells seemed to be caused by soluble enhancing factors secreted by the exudate cells. The exudate cells maximally secreted the enhancing factors when harvested from mice on day 4 of the induction period following the injection of casein. A semi-continuous culture of the hybridoma demonstrated the applicability of the culture supernatant to enhance antibody production by producing a two-fold increase over the control for seven days when supplemented with the supernatant at 5%. Significant amounts of interleukin-6 were detected in culture supernatant of the exudate cells. Interleukin-6 obtained from other sources enhanced the antibody productivity two-fold when added to the hybridoma culture at the concentration of 5 unit/ml. Interleukin-6, therefore, is expected to be one of the principal antibody enhancing factors secreted by the exudate cells. Other interleukins examined, that is, interleukin-1 to-5 did not enhance the antibody productivity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00350915
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Interleukin-6 is antiproliferative to a mouse hybridoma cell line and promotive for its antibody productivity (1992)
    Springer
    Cytotechnology 10 (1992), S. 15-23 
    Details
    ISSN: 1573-0778
    Keywords: antibody productivity ; growth suppression ; hybridoma ; interleukin-6 ; specific productivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Monoclonal antibody production by hybridoma cells at moderately slowed growth states would be favorable for commercial scale production since cells can devote their resources to performing the differentiated function, immunoglobulin production. We found that a purified recombinant human interleukin-6, which had been reported to support or stimulate proliferation of B cell hybridoma/plasmacytoma cells, suppressed growth of a hybridoma cell line in serum-free medium. In the presence of the interleukin, the growth-suppressed cells were viable for remarkably long periods in batch culture, and after removal of the interleukin from the culture medium, they started to proliferate at their normal growth rate. As the concentration of the interleukin increased in the culture, the growth rate decreased and the specific antibody productivity (antibody production rate per cell) increased to 5-fold of control at 10 U ml−1 (2 ng ml−1) of the interleukin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00376096
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  • 4
    Electronic Resource
    Electronic Resource
    Growth rate suppression of cultured mammalian cells enhances protein productivity (1994)
    Springer
    Cytotechnology 15 (1994), S. 57-64 
    Details
    ISSN: 1573-0778
    Keywords: Enhancement of protein production ; growth suppression ; cell differentiation ; mammalian cell culture ; mRNA stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Suppression of proliferation of cells which contain stable or stabilized mRNA coded for a protein to be produced, a partial mimic of cell differentiation, was examined for enhancing protein production by cultured mammalian cells. Hybridoma 2E3 cells which were adapted to be interleukin-6 sensitively growth-suppressed accumulated the mRNA of IgG1 which is reported stable, and IgG1 production rate increased as a result when their growth was suppressed with interleukin-6. A myeloma cell line was similarly adapted; the obtained myeloma cells can be used as host cells for enhancing production of exogenous proteins by suppressing growth with interleukin-6. Temperature-sensitively growth-suppressible mutants of mouse mammary carcinoma FM3A were transfected with cDNA of IgM λ1 chain and cultured at nonpermissive temperature to enhance production of λ1. Addition of various growth-suppressive reagents to culture medium was studied for finding methods suitable for suppressing growth while maintaining high cell viability. Caffeine yielded the best results among these reagents. Deprivation of various growth-supporting components in culture medium was also tested; simultaneous deprivation of insulin and transferrin viably suppressed growth of hybridoma 2E3 cells, resulting in enhanced antibody productivity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00762379
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    Paper (German National Licenses)
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