ISSN:
1617-4623
Keywords:
Key words Antibiotic
;
bldA
;
Thioesterase
;
Transcriptional regulator
;
Polyketide biosynthesis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract In a search for activators of secondary metabolism we isolated a 12.6-kb DNA fragment from a genomic library of Streptomyces ambofaciens NRRL 2240 (the spiramycin producer ). Sequencing of 6 kb of the cloned fragment revealed a cluster of four ORFs (ORF1–4) whose deduced products showed similarities to those of other genes involved in polyketide biosynthesis, including a pathway-specific regulatory gene of the SARP family. The results of insertional inactivation of some of the cloned genes clearly indicate that the isolated cluster does not code for spiramycin production, suggesting that some other polyketide compound might well be produced by this strain.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004380051135
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