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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of rapid methods and automation in microbiology 1 (1992), S. 0 
    ISSN: 1745-4581
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The speed of ATP bioluminescence assays makes them very useful for assessing product quality and efficiency of sanitation at food plants. the suitability of a new method for evaluating the quality of raw milk was compared with plate count. A good correlation was obtained between the ATP method and plate count for milks with counts above 1 × 104 cfu/ml. the combination of ATP bioluminescence with preincubation procedures was investigated for predicting the shelf-life of pasteurized milk. the best correlations were obtained when milks were preincubated at 15C for 25 h or at 21C for 25 h in the presence of an inhibitor system to prevent Gram-positive bacterial growth.Hygiene monitoring using bioluminescence gave a better indication of surface cleaniness than conventional swab counts. the reasons for the discrepancies in a small percentage of cases are discussed.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food safety 16 (1996), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The antibacterial activity of combinations of lysozyme, monolaurin (ML), triglycerol 1,2 laurate (TGL) and butylated hydroxyanizole (BHA) against 7 Gram-positive and 8 Gram-negative bacteria was studied at different pH, NaCl and EDTA concentrations by the spiral gradient end point test. The inhibitory effect of lysozyme in combination with ML was slightly greater for Gram-positive than for Gram-negative bacteria, but their combined effect was not markedly more inhibitory than ML alone. Lysozyme and TGL together were only inhibitory at low pH and high NaCl concentrations in the presence of EDTA. There was an increase in inhibition when lysozyme and BHA were combined. For Gram-positive bacteria, inhibition by ML and BHA together was more marked than when either was present singly. However, ML decreased the antibacterial activity of BHA against Gram-negative bacteria. Similarly, TGL was antagonistic to BHA action against both Gram-positive and Gram-negative bacteria. In general, the inhibition produced by all combinations was greater as the pH decreased and the NaCl concentration increased, especially in the presence of EDTA.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food safety 19 (1999), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The antifungal activity of butylated hydroxyanizole (BHA) and lysozyme alone and combined with ethylenediaminetetraacetic acid (EDTA), at different pH (3.5, 5.6 and 7), and different NaCl concentrations (0.5 to 20%) was screened on 12 fungi. The spiral gradient end point test was used to determine the minimum inhibitory concentration (MIC). BHA was effective against all the fungi tested in the range of 〈 12 to 460 μg/mL. In the presence of equal amounts of EDTA, the inhibitory effect was amplified (inhibition occurred between 〈 12 to 200 μg/mL). The inhibition was more pronounced at pH 5.6 and at higher NaCl concentrations especially when EDTA was present. Lysozyme alone was only effective against Fusarium graminearum PM162 and Aspergillus ochraceus MM 184, but was inhibitory for all fungi, except Candida lipolitica 1591, in the presence of EDTA. The inhibition was greatest at pH 5.6 and higher NaCl concentrations, especially when EDTA was present. These studies show that the antifungal properties of lysozyme and BHA can be greatly influenced by environmental factors.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of rapid methods and automation in microbiology 4 (1995), S. 0 
    ISSN: 1745-4581
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Bioluminescence has gained acceptance as a rapid technique for quickly assessing the cleanliness of food contact surfaces, and a variety of commercial systems are available. However, with advances in reagents and instrumentation and knowledge of the genetics of the light reaction, the potential of bioluminescence as a tool in food microbiology is only now beginning to be realized. This short review outlines the areas where this technology may have application in the future.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of rapid methods and automation in microbiology 3 (1995), S. 0 
    ISSN: 1745-4581
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In order to simplify plate count procedures used in the food industry, a number of strategies has been adopted. The preparation and handling of media have been simplified with the advent of bench top sterilizers and automated plate pourers. Inoculation of plates using spiral platers have decreased the need for dilutions and special devices have been developed to make plate counts easier. Plates can be counted automatically using image analysis instrumentation and, in some instances, results can be obtained faster by the adoption of modified incubation conditions.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of rapid methods and automation in microbiology 3 (1994), S. 0 
    ISSN: 1745-4581
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Many methods have been developed to determine microbial levels in food products and these include ATP bioluminescence, hydrophobic grid membrane filtration (HGMF), impediometry and turbidimetry. A comparison of these techniques for detecting microbial levels in chicken carcass rinses was conducted.Only the ATP bioluminescence assay and the HGMF system showed a good correlation with plate counts (r = 0.82 and r = 0.83, respectively). The repeatability of these methods was acceptable. There was also a significant correlation between results obtained with two turbidimetric methods and HGMF as well as between HGMF and ATP bioluminescence data. However, only the ATP bioluminescence assay was able to provide results of microbial levels on a realtime basis (within 10 min). This would be beneficial for HACCP (Hazard Analysis of Critical Control Points) based programs.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food safety 16 (1996), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effect of pH (5 to 7), NaCl concentration (0.5 to 5%) and chelating agents on the antibacterial activity of lysozyme and butylated hydroxyanizole (BHA) was investigated by the Spiral Gradient End Point test. Of all the bacteria tested (including 7 Gram-positive and 8 Gram-negative organisms), lysozyme was active only against Lactococcus lactis NCK 400. When EDTA was added, lysozyme was inhibitory for all of the Gram-positive and 4 of the Gram-negative species. Butylated hydroxyanizole was active against all the organisms tested, except for 2 pseudomonads, but these were inhibited when EDTA was added. The inhibition effected by lysozyme and BHA, both in the presence and absence of EDTA, was greatest at low pH and high NaCl concentrations. The inhibition by lysozyme was unaffected by other chelators (sodium citrate and monoglycerol citrate). However, sodium citrate promoted the inhibitory effect of BHA towards Gram-negative bacteria and monoglycerol citrate lowered the minimum inhibitory concentration required by BHA to inhibit 5 of the 7 Gram-positive bacteria by 〈inlineGraphic alt="geqslant R: gt-or-equal, slanted" extraInfo="nonStandardEntity" href="urn:x-wiley:01496085:JFS59:ges" location="ges.gif"/〉 10%.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of rapid methods and automation in microbiology 3 (1995), S. 0 
    ISSN: 1745-4581
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Over the last four decades, many novel methods for the detection and enumeration of microorganisms in foods have been developed. This article briefly reviews some of these and discusses some of their applications.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Physics Letters A 64 (1977), S. 309-310 
    ISSN: 0375-9601
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 205 (2001), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The effect of environmental and chemotactic stimuli was assessed using a fusion between the Campylobacter jejuni flaAσ28 promoter and a promoterless luxCDABE cassette on pRYluxCDABE. Environmental stimuli relevant to the enteric environment were found to modulate flaA promoter activity. pH, bovine bile, deoxycholate, l-fucose and osmolarity up-regulated the flaA promoter while viscosity resulted in down-regulation. C. jejuni chemotactic effectors, including the amino acids aspartate and glutamate and the organic acids citrate, fumarate, α-ketoglutarate and succinate up-regulated the flaA promoter. Proline, which does not elicit a chemotactic response, up-regulated the flaA promoter.
    Type of Medium: Electronic Resource
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