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  • 1
    Electronic Resource
    Electronic Resource
    Detection of tomato spotted wilt virus and transmission by Frankliniella occidentalis in France (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 40 (1991), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Tomato spotted wilt virus (TSWV) was isolated from pepper, tomato, eggplant, broad bean, lettuce, basil, chrysanthemum, aster, New Guinea impatiens, anemone and gloxinia plants. Virus identification was based on host range, vector transmission, serology and electron microscopy. TSWV was readily detected by ELISA in naturally or artificially infected cultivated or weed plants. The virus was also detected in individual F. occidentalis thrips. The spread of TSWV in vegetable and ornamental plants in greenhouses and/or in the open is related to the close relationship of the virus with the vector.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3059.1991.tb02389.x
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  • 2
    Electronic Resource
    Electronic Resource
    Evidence for two distinct subgroups of Alfalfa mosaic virus (AMV) from France and Italy and their relationships with other AMV strains (2000)
    Springer
    Archives of virology 145 (2000), S. 2659-2667 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary.  The nucleotide sequence of the putative coat protein open reading frame of seven previously uncharacterized AMV strains from Italy and France was determined and aligned with comparable sequences of other AMV strains (425 L, 425 M, YSMV, S, VRU, 15/64 and Da). The data set of AMV sequences was used to determine phylogenetic relationships by both a stochastic (stationary Markov model) and a deterministic method (maximum-parsimony) of analysis. The topology of the trees obtained with the two methods was essentially the same showing that all AMV strains clustered in two monophyletic groups. Close clustering of Italian strains in subgroup I and of French strains in subgroup II seems to suggests the effect of geographic distinctiveness of evolutionary dynamics of these AMV strains. This separation did not correlate with differences in host range or symptoms (necrotic or non necrotic) induced in tomato but rather it reflected variations in the amino acid sequence of their CP, which might be related to structural properties of virus particles. A simple and rapid procedure based on the reverse transcriptase-polymerase chain reaction (RT-PCR) followed by ezymatic digestion (RFLP) was developed to identify and classify AMV isolates into the two subgroups. The method applied to a number of other AMV isolates from Italy and France supported their division in two distinct subgroups. This RT-PCR RFLP method may be useful way to investigate the dynamics of AMV populations in nature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007050070014
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  • 3
    Electronic Resource
    Electronic Resource
    Recherches sur les microorganismes de type mycoplasme dans les cicadelles vectrices et dans les vegetaux atteints de jaunisses (1968)
    Springer
    Entomologia experimentalis et applicata 11 (1968), S. 470-474 
    Details
    ISSN: 1570-7458
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Summary The microorganisms causing the diseases Clover phyllody, Stolbur C and Stolbur SM were studied in the leafhopper Euscelis plebejus, and in the plants Trifolium repens, Cuscuta subinclusa, Datura stramonium and Solanum lycopersicum, by electron microscope examination of ultrathin sections and purified suspensions. In infected host plants and insects the following components were distinguished: large, ovoid, amoeboid or elongated bodies, well-contrasted isolated or chained microbodies, minute filaments, and degenerate elements. Some of these bodies resemble mycoplasma. In the leafhoppers it is possible that the degeneration of these mycoplasma-like microorganisms is due to an immunological reaction, which may explain why aged vectors do not transmit the diseases. In plants, the degeneration of the microorganisms was also observed. This loss may partly account for the occasional complete, or temporary, recovery of diseased plants.
    Notes: Résumé La phyllodie du trèfle, le Stolbur C et le Stolbur SM ont été étudiés chez la cicadelle Euscelis plebejus, les plantes Trifolium repens, Cuscuta subinclusa, Datura stramonium et Solanum lycopersicum en microscopie électronique, sur coupes ultrafines et après purification des agents infectieux. Dans les différents hôtes infectés, on distingue des éléments de grande taille, de forme ovoïde, amiboïde ou allongée, des corpuscules très contrastés, isolés ou en chaînettes, de fins filaments et des élements dégénérescents. Chez les cicadelles, la dégénérescence de ces microorganismes est probablement due à une réaction immunologique qui expliquerait la perte régulière du pouvoir infectieux de ces vecteurs. Chez les plantes, cette dégénérescence peut en partie rendre compte des phénomènes de rétablissements définitifs ou temporaires que manifestent parfois certains végétaux atteints de ces maladies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00364578
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  • 4
    Electronic Resource
    Electronic Resource
    Hypersensitive resistance to tomato spotted wilt virus in three Capsicum chinense accessions is controlled by a single gene and is overcome by virulent strains (1997)
    Springer
    Euphytica 94 (1997), S. 45-52 
    Details
    ISSN: 1573-5060
    Keywords: allelism test ; Capsicum chinense ; hypersensitive resistance ; pepper ; tomato spotted wild virus ; virus evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The resistant reaction to tomato spotted wilt virus (TSWV) was found to be determined by a single dominant gene in three Capsicum chinense Jacq. accessions (‘PI 152225’, ‘PI 159236’, ‘7204’). Allelism studies indicated that all C. chinense lines bear the same allele located at the Tsw locus. All the inoculated plants in the allelism tests displayed a resistant hypersensitive phenotype characterized by necrotic local lesions followed by abscission of the inoculated organ. However, a small proportion of them showed late systemic infection. Nine TSWV isolates obtained from these individual plants with systemic symptoms were backinoculated to the three resistant parents. All isolates were able to infect systemically all the resistant accessions without inducing local necrotic lesions. Serological analysis confirmed that these nine viral isolates belong to the TSWV species (serogroup I). Consequently, the susceptible plants in the allelism tests could not be interpreted as possessing a recombinant genotype because of the virulence change in the viral strain. Hobbs et al. (1994) already reported the existence of TSWV pathotypes overcoming the resistance of C. chinense resistant accessions. Practical consequences for pepper breeding associated with the emergence of these resistance-breaking isolates are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1002997522379
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  • 5
    Electronic Resource
    Electronic Resource
    Evaluation and inheritance of the Lycopersicon hirsutum resistance against potato virus Y (1995)
    Springer
    Euphytica 86 (1995), S. 219-226 
    Details
    ISSN: 1573-5060
    Keywords: ELISA ; Lycopersicon hirsutum ; mechanisms of resistance ; potato virus Y ; tomato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Potato virus Y (PVY) infects most Solanaceous crops grown in Mediterranean countries in open fields and in greenhouses. Necrogenic strains, which have been isolated from diseased tomatoes in France since the 1980's, seriously cause yield and quality loss of tomato fruits. Lycopersicon hirsutum PI 247087 was found to be resistant to PVY. Virus could not be detected in inoculated leaves by ELISA and/or by back-inoculation on susceptible plants. This resistance was efficient against the 16 tested isolates or strains. Temperature and inoculum concentration did not affect its expression. All the F1 plants of (Momor × PI 247087), (PI 134417 × PI 247087) and (PI 247087 × PI 134417) had symptom scores and ELISA values similar to those of the susceptible parents. The mechanism of resistance could be immunity-like or inhibition of virus migration from cell to cell. The resistance of L. hirsutum PI 247087 appeared to be governed by two independent recessive genes. In a few F2 plants of (PI 134417 × PI 247087) and F2 (Momor × PI 247087), virus was able to multiply in the inoculated leaves but could not establish a systemic infection. This finding may suggest a mechanism which interfers with the long distance migration of the virus in the plant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016359
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