ISSN:
1432-0584
Keywords:
Key words Indian ALL
;
MRP
;
MDR1
;
GSTπ
;
GSTμ
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract In order to investigate the phenomenon of multidrug resistance as a possible mechanism for poor response to treatment in patients with acute lymphoblastic leukemia (ALL) from India, a series of 32 cases of de novo untreated ALLs were analyzed by a cDNA-PCR approach to estimate the relative mRNA levels of the MDR-associated genes encoding MDR1, MRP, GSTπ, and GSTμ. The expression of β2 microglobulin served as an internal standard. Quantifiable transcripts were observed in 20 patients for MRP, in 5 for MDR1, in 24 for GSTπ, and in 19 for GSTμ. The values ranged from undetectable to 132% of the control A549 cell line for MRP, undetectable to 49% of the HL60/DNR control cell line for MDR1, undetectable to 268% of A549 control cell line for GSTπ, and undetectable to 247% of A549 control cell line for GSTμ mRNA. Increased MRP levels were associated with increased GSTπ and GSTμ levels (p〈0.01 for both), and increased levels of MDR1 were associated with increased GSTπ levels (p〈0.05). The present observations showed no correlation between the MDR1 and MRP values with treatment outcome, in terms of either achieving a complete remission or predilection to early relapse. In view of some recent studies that envisage MRP as an energy-dependent pump involved in the efflux of GSH conjugates, the simultaneous up-regulation of transcription of all these genes might well be part of an integrated detoxification response that has been switched on after exposure to an environmental stress.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002770050388
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