ZIB

Treffer pro Seite

Treffer 1 - 2 | 2 Treffer

Sortierung

Digitale Medien

Dihydrofolate reductase: A potential drug target in trypanosomes and leishmania (1998)

Zuccotto, Fabio ; Martin, Andrew C.R ; Laskowski, Roman A. ; [weitere]

Springer

Journal of computer aided molecular design 12 (1998), S. 241-257

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 1573-4951

Schlagwort(e): comparative molecular modelling ; dihydrofolate reductase ; drug design ; protein structure

Quelle: Springer Online Journal Archives 1860-2000

Thema: Chemie und Pharmazie

Notizen: Abstract Dihydrofolate reductase has successfully been used as a drug target in the area of anti-cancer, anti-bacterial and anti-malarial chemotherapy. Little has been done to evaluate it as a drug target for treatment of the trypanosomiases and leishmaniasis. A crystal structure of Leishmania major dihydrofolate reductase has been published. In this paper, we describe the modelling of Trypanosoma cruzi and Trypanosoma brucei dihydrofolate reductases based on this crystal structure. These structures and models have been used in the comparison of protozoan, bacterial and human enzymes in order to highlight the different features that can be used in the design of selective anti-protozoan agents. Comparison has been made between residues present in the active site, the accessibility of these residues, charge distribution in the active site, and the shape and size of the active sites. Whilst there is a high degree of similarity between protozoan, human and bacterial dihydrofolate reductase active sites, there are differences that provide potential for selective drug design. In particular, we have identified a set of residues which may be important for selective drug design and identified a larger binding pocket in the protozoan than the human and bacterial enzymes.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1023/A:1016085005275

Permalink

Bibliothek	Standort	Signatur	Band/Heft/Jahr	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext

Digitale Medien

Accessing the Kabat antibody sequence database by computer (1996)

Martin, Andrew C.R.

New York, NY : Wiley-Blackwell

Proteins: Structure, Function, and Genetics 25 (1996), S. 130-133

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 0887-3585

Schlagwort(e): immunoglobulin ; sequence comparison ; cloning ; complementarity determining regions ; alignment ; Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Medizin

Notizen: The Kabat antibody sequence database has for many years been the primary site for depositing sequence information on antibodies and other proteins of immunological interest. The chief drawback of this database has been that it has only been available in the form of a printed book (Kabat et al., Sequences of Proteins of Immunological Interest, 1991). These data have recently become available on the global computer Internet, but no method of searching the data has, as yet, been provided. Here, the development of a specialized database program for accessing the antibody data is described. This database software has been made accessible over the World Wide Web, together with a program which allows a novel antibody sequence to be tested against the Kabat sequence database, to identify unusual features of an antibody sequence which may represent cloning artifacts or sequencing errors. © 1996 Wiley-Liss, Inc.

Zusätzliches Material: 1 Ill.